UR: Physiology and Aquatic Environment, Department of Biology, Faculty of Sciences of Tunis, Univ. Tunis El Manar, 2092 Tunis, Tunisia.
UR: Physiology and Aquatic Environment, Department of Biology, Faculty of Sciences of Tunis, Univ. Tunis El Manar, 2092 Tunis, Tunisia.
Ecotoxicol Environ Saf. 2019 Mar;169:516-522. doi: 10.1016/j.ecoenv.2018.11.051. Epub 2018 Nov 23.
Mercury is one of the most harmful pollutant that threat marine biota. This study assessed the Hg impact on the fatty acid (FA) composition and the antioxidant statues in Holothuria forskali body wall tissue. Specimens were exposed to HgCl graded doses (40, 80 and 160 μg L) for 96 h. A decrease in linoleic, arachidonic and eicosapentaenoic acid levels and an increase of docosahexaenoic acid were mainly observed at the nominal tested dose. The exposure to the upper dose promoted oxidative stress with an increase of malondialdehyde, hydrogen peroxide, advanced oxidation protein product, glutathione and non-protein thiols levels. Moreover, a decrease in catalase and an increase in superoxide dismutase and glutathione peroxidase activities were observed. Yet, an increase of the metallothionein level was registered in all treated groups. This study confirmed the Hg toxicity on the redox statue of H. forskali and highlighted the usefulness of the FA composition as an early sensitive bioindicators.
汞是威胁海洋生物群的最有害污染物之一。本研究评估了 Hg 对海参体壁组织中脂肪酸 (FA)组成和抗氧化状态的影响。将标本暴露于 HgCl 不同剂量（40、80 和 160μg/L）96 小时。在名义测试剂量下，主要观察到亚油酸、花生四烯酸和二十碳五烯酸水平下降，二十二碳六烯酸水平升高。暴露于高剂量会引起氧化应激，导致丙二醛、过氧化氢、高级氧化蛋白产物、谷胱甘肽和非蛋白巯基水平升高。此外，还观察到过氧化氢酶活性降低，超氧化物歧化酶和谷胱甘肽过氧化物酶活性增加。然而，所有处理组的金属硫蛋白水平都有所增加。本研究证实了 Hg 对 H. forskali 氧化还原状态的毒性，并强调了 FA 组成作为早期敏感生物标志物的有用性。
