UR: Physiology and Aquatic Environment, Department of Biology, Faculty of Sciences of Tunis, University Tunis El Manar, Tunis, Tunisia.
BVBGR-LR11ES31, Higher Institute of Biotechnology - Sidi Thabet, Biotechpole Sidi Thabet, University Manouba, Ariana, Tunisia.
Drug Chem Toxicol. 2020 May;43(3):287-297. doi: 10.1080/01480545.2018.1524475. Epub 2018 Dec 17.
Mercury (Hg) is among the most deleterious contaminant in the aquatic environment and presents a serious risk to humans and ecosystems. This study evaluated the effects of Hg on oxidative stress biomarkers, DNA integrity and histological structure of the respiratory tree of exposed to different concentrations of mercury chloride HgCl (0.04, 0.08 and 0.16 mg L) for 96 h. Exposure of to Hg led to oxidative stress with an increase in Malondialdehyde (MDA), hydrogen peroxide (HO), advanced oxidation protein product (AOPP) and protein carbonyls (PCO) levels in the treated groups. Alteration of the antioxidant system was also confirmed by the significant increase in glutathione (GSH), nonprotein thiol (NPSH) and vitamin C contents. Moreover, the enzymatic activity of superoxide dismutase (SOD), Glutathione peroxidase (GPX) and Catalase (CAT) increased significantly. Our research revealed that total Metallothionein (MTs) content enhanced in a dose-dependent manner. Interestingly, the exposure to this metal provoked a decrease in Acetylcholinesterase (AChE) activity. Hg genotoxicity was further evidenced by a random DNA degradation that was observed in the treated groups. The histopathological findings confirmed the biochemical results. Overall, our results indicated that mercury-induced genotoxicity, oxidative damage and histopathological injuries in the respiratory tree of
汞（Hg）是水生环境中最具危害性的污染物之一，对人类和生态系统构成严重威胁。本研究评估了氯化汞（HgCl）暴露于不同浓度（0.04、0.08 和 0.16 mg/L）96 小时对呼吸树的氧化应激生物标志物、DNA 完整性和组织学结构的影响。Hg 暴露导致呼吸树发生氧化应激，丙二醛（MDA）、过氧化氢（HO）、高级氧化蛋白产物（AOPP）和蛋白质羰基（PCO）水平在处理组中升高。抗氧化系统的改变也通过谷胱甘肽（GSH）、非蛋白巯基（NPSH）和维生素 C 含量的显著增加得到证实。此外，超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）和过氧化氢酶（CAT）的酶活性显著增加。我们的研究表明，总金属硫蛋白（MTs）含量呈剂量依赖性增加。有趣的是，暴露于这种金属会导致乙酰胆碱酯酶（AChE）活性下降。Hg 的遗传毒性进一步通过在处理组中观察到的随机 DNA 降解得到证实。组织病理学结果证实了生化结果。总的来说，我们的结果表明，汞诱导的呼吸树的遗传毒性、氧化损伤和组织病理学损伤。
