Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Lab of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing, 100190, China.
Angew Chem Int Ed Engl. 2019 Jan 14;58(3):796-800. doi: 10.1002/anie.201812582. Epub 2018 Dec 7.
Prototypes of natural biosystems provide opportunities for artificial biomimetic systems to break the limits of natural reactions and achieve output control. However, mimicking unique natural structures and ingenious functions remains a challenge. Now, multiple biochemical reactions were integrated into artificially designed compartments via molecular assembly. First, multicompartmental silica nanoparticles with hierarchical structures that mimic the chloroplasts were obtained by a templated synthesis. Then, photoacid generators and ATPase-liposomes were assembled inside and outside of silica compartments, respectively. Upon light illumination, protons produced by a photoacid generator in the confined space can drive the liposome-embedded enzyme ATPase towards ATP synthesis, which mimics the photophosphorylation process in vitro. The method enables fabrication of bioinspired nanoreactors for photobiocatalysis and provides insight for understanding sophisticated biochemical reactions.
天然生物系统的原型为人工仿生系统提供了突破自然反应限制和实现输出控制的机会。然而，模仿独特的自然结构和巧妙的功能仍然是一个挑战。现在，通过分子组装，将多种生化反应整合到人工设计的隔室中。首先，通过模板合成获得了具有类叶绿体结构的多级结构的多隔室硅纳米粒子。然后，分别在二氧化硅隔室的内外组装光酸发生器和 ATP 酶脂质体。在光照下，受限空间中光酸发生器产生的质子可以驱动嵌入脂质体的酶 ATP 酶向 ATP 合成方向移动，从而在体外模拟光磷酸化过程。该方法可用于制备用于光生物催化的仿生纳米反应器，并为深入了解复杂的生化反应提供了思路。
