Dong Xiaohua, Zhao Yingmin, Huang Yun, Yu Lingling, Yang Xiaojing, Gao Feng
Department of Pediatric, Jingjiang People's Hospital Affiliated to Yangzhou University, Jingjiang, Jiangsu, China.
J Cell Biochem. 2019 May;120(5):8499-8509. doi: 10.1002/jcb.28138. Epub 2018 Nov 26.
Long noncoding RNAs (lncRNAs) have been shown to participate in many biological processes. To investigate the expression profiles of lncRNAs and their potential functions in neonatal hypoxic-ischemic encephalopathy (HIE), we detected the lncRNA and messenger RNA (mRNA) expression in the peripheral blood samples from HIE patients and controls using a microarray. A total of 376 lncRNAs and 126 mRNAs were differentially expressed between the HIE and the non-HIE samples (fold change > 2). Quantitative real-time polymerase chain reaction was used to validate the microarray data. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis were performed to determine the gene function. Furthermore, the lncRNA-mRNA coexpression network was generated to predict the potential targets of lncRNAs. In conclusion, our study first demonstrated the differential expression profiles of lncRNAs in the whole blood of infants with HIE and may provide a new view of the distinct lncRNA functions in HIE.
长链非编码RNA（lncRNAs）已被证明参与许多生物学过程。为了研究lncRNAs的表达谱及其在新生儿缺氧缺血性脑病（HIE）中的潜在功能，我们使用微阵列检测了HIE患者和对照组外周血样本中的lncRNA和信使RNA（mRNA）表达。HIE样本和非HIE样本之间共有376个lncRNAs和126个mRNAs差异表达（倍数变化>2）。采用定量实时聚合酶链反应验证微阵列数据。进行基因本体富集分析和京都基因与基因组百科全书通路分析以确定基因功能。此外，构建lncRNA-mRNA共表达网络以预测lncRNAs的潜在靶标。总之，我们的研究首次证明了HIE婴儿全血中lncRNAs的差异表达谱，并可能为HIE中lncRNAs的独特功能提供新的视角。
