Lee Seok Hee, Oh Hyun Ju, Kim Min Jung, Kim Geon A, Setyawan Erif Maha Nugraha, Ra Kihae, Abdillah Dimas Arya, Lee Byeong Chun
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea.
Reprod Domest Anim. 2018 Nov;53 Suppl 3:133-138. doi: 10.1111/rda.13358.
Since the generation of world's first cloned dog, Snuppy, in 2005, somatic cell nuclear transfer (SCNT) in dogs has been widely applied for producing several kinds of dogs with specific objectives. Previous studies have demonstrated that cloned dogs show normal characteristics in growth, blood parameters and behavioural aspect. Also, canine SCNT technique has been applied to propagate working dogs with excellent abilities in fields such as assistance of disabled people, drugs detection and rescue activity. Because dogs have similar habituation properties and share many characteristics including anatomic and physiological aspects with humans, they are also primary candidates for human disease models. Recently, transgenic dogs that express red fluorescent protein gene constitutively and green fluorescent protein gene conditionally have been generated. In addition, transgenic dogs with an overexpression of peroxisome proliferator-activated receptor-alpha in specific muscles were generated to enhance physical performance. In 2017, Snuppy was recloned with markedly increased pregnancy and delivery rates compared to the statistics from when Snuppy was first cloned. Such striking improvements in the cloning of dogs using SCNT procedures suggest that dog cloning could be applied in many fields of biomedical science for human diseases research, and the application of cloning is no longer science fiction.
自2005年世界上第一只克隆狗斯纳皮诞生以来，狗的体细胞核移植（SCNT）已被广泛应用于培育具有特定目标的多种狗。先前的研究表明，克隆狗在生长、血液参数和行为方面表现出正常特征。此外，犬类体细胞核移植技术已被应用于繁殖在协助残疾人、毒品检测和救援活动等领域具有出色能力的工作犬。由于狗具有与人类相似的习性，并且在解剖学和生理学等许多方面具有共同特征，它们也是人类疾病模型的主要候选对象。最近，已经培育出了组成型表达红色荧光蛋白基因和条件性表达绿色荧光蛋白基因的转基因狗。此外，还培育出了在特定肌肉中过表达过氧化物酶体增殖物激活受体α的转基因狗，以提高体能。2017年，斯纳皮被再次克隆，与首次克隆斯纳皮时的统计数据相比，怀孕和分娩率显著提高。使用体细胞核移植程序克隆狗的这些显著改进表明，狗克隆可应用于生物医学科学的许多领域以进行人类疾病研究，并且克隆的应用不再是科幻小说。
