Yamaki Tomohiro, Onodera Shinji, Uchida Tomoki, Ozaki Yoshihiro, Yokoyama Kazuaki, Henmi Haruko, Kamezawa Mizuho, Hayakawa Miyoko, Itou Daisuke, Oka Nobuo, Odaki Masaru, Iwadate Yasuo, Kobayashi Shigeki
Division of Neurosurgery, Rehabilitation Center for Traumatic Apallics Chiba, National Agency for Automotive Safety and Victims' Aid; Division of PET imaging, Rehabilitation Center for Traumatic Apallics Chiba, National Agency for Automotive Safety and Victims' Aid;
Division of PET imaging, Rehabilitation Center for Traumatic Apallics Chiba, National Agency for Automotive Safety and Victims' Aid.
J Vis Exp. 2018 Nov 9(141). doi: 10.3791/58641.
Patients with severe traumatic brain injury (sTBI) have difficulty knowing whether they are accurately expressing their thoughts and emotions because of disorders of consciousness, disrupted higher brain function, and verbal disturbances. As a consequence of an insufficient ability to communicate, objective evaluations are needed from family members, medical staff, and caregivers. One such evaluation is the assessment of functioning brain areas. Recently, multimodal brain imaging has been used to explore the function of damaged brain areas. [F]-fluorodeoxyglucose positron emission tomography-computed tomography ([F]FDG-PET/CT) is a successful tool for examining brain function. However, the assessment of brain glucose metabolism based on [F]FDG-PET/CT is not standardized and depends on several varying parameters, as well as the patient's condition. Here, we describe a series of semiquantitative assessment protocols for a region-of-interest (ROI) image analysis using self-produced [F]FDG tracers in patients with sTBI. The protocol focuses on screening the participants, preparing the [F]FDG tracer in the hot lab, scheduling the acquisition of [F]FDG-PET/CT brain images, and measuring glucose metabolism using the ROI analysis from a targeted brain area.
患有严重创伤性脑损伤（sTBI）的患者由于意识障碍、高级脑功能受损和言语障碍，难以知晓自己是否准确表达了想法和情感。由于沟通能力不足，需要家庭成员、医护人员和护理人员进行客观评估。其中一种评估是对脑功能区域的评估。最近，多模态脑成像已被用于探索受损脑区的功能。[F] - 氟脱氧葡萄糖正电子发射断层扫描 - 计算机断层扫描（[F]FDG - PET/CT）是一种用于检查脑功能的成功工具。然而，基于[F]FDG - PET/CT的脑葡萄糖代谢评估并不标准化，它取决于多个不同参数以及患者的病情。在此，我们描述了一系列使用自行制备的[F]FDG示踪剂对sTBI患者进行感兴趣区域（ROI）图像分析的半定量评估方案。该方案重点在于筛选参与者、在热室中制备[F]FDG示踪剂、安排[F]FDG - PET/CT脑图像采集以及使用来自目标脑区的ROI分析测量葡萄糖代谢。
