Kim Dong Eun, Davalos Albert R
Buck Institute for Research on Aging, Novato, CA, USA.
Methods Mol Biol. 2019;1896:71-81. doi: 10.1007/978-1-4939-8931-7_8.
Senescent cells secrete diverse array of proteins. One group of proteins, damage-associated molecular pattern (DAMP) proteins exhibit relocalization from inside to outside the cell. High Mobility Group Box 1 protein (HMGB1) is the founding DAMP member. HMGB1 relocalization from the nucleus provides a molecular signature during senescence. We provide distinct molecular techniques (immunofluorescence, immunohistochemistry, and Western blot assays) to assess HMGB1 relocalization during the initial stages of senescence.
衰老细胞会分泌多种蛋白质。其中一组蛋白质，即损伤相关分子模式（DAMP）蛋白，会从细胞内部重新定位到细胞外部。高迁移率族蛋白B1（HMGB1）是DAMP蛋白的首个成员。HMGB1从细胞核的重新定位是衰老过程中的一种分子特征。我们提供了独特的分子技术（免疫荧光、免疫组织化学和蛋白质印迹分析）来评估衰老初期HMGB1的重新定位。
