Wiley Christopher D, Davis Sonnet, Ramanathan Arvind
Buck Institute for Research on Aging, Novato, CA, USA.
Methods Mol Biol. 2019;1896:139-147. doi: 10.1007/978-1-4939-8931-7_13.
Senescent cells display altered metabolic signatures that can either be causal for aspects the senescent phenotype, or act as biomarkers for senescence. Steady state levels of multiple metabolites change with senescence, and can be detected using analytical methods. Here, we describe a liquid chromatography-mass spectrometry (LC-MS) method for detecting altered metabolites from cultured senescent cells.
衰老细胞表现出改变的代谢特征，这些特征要么是衰老表型某些方面的原因，要么作为衰老的生物标志物。多种代谢物的稳态水平会随着衰老而变化，并且可以使用分析方法进行检测。在这里，我们描述了一种用于检测培养的衰老细胞中代谢物变化的液相色谱 - 质谱（LC-MS）方法。
