Mall Isaac, Musteata Florin Marcel
Department of Pharmaceutical Sciences, Albany College of Pharmacy & Health Sciences, 106 New Scotland Avenue, Albany, NY 12208, USA.
Bioanalysis. 2019 Jan;11(2):61-72. doi: 10.4155/bio-2018-0138. Epub 2018 Nov 26.
Transdermal analysis is proposed for vitamin D and its hydroxylated metabolite to overcome problems associated with blood analysis.
Vitamin D was extracted directly from skin with solid patches and liquid phases. Deuterium-labeled vitamin D was added to the extraction solutions to compensate for variability and accurately determine the rate of transdermal transfer. Of the different extraction solvents tested, 50:50 octanol:isopropanol showed the best results, with an accuracy of 115% and reproducibility better than 30%.
The research shows that transdermal route can be used for analysis of vitamin D in porcine skin. When microneedles are used, accurate measurements were obtained in 1 h. With intact skin, the highest accuracy was obtained when extraction was done for 2 h.
提出对维生素D及其羟基化代谢物进行透皮分析，以克服与血液分析相关的问题。
用固体贴片和液相从皮肤中直接提取维生素D。将氘标记的维生素D添加到提取溶液中，以补偿变异性并准确测定透皮转移速率。在所测试的不同提取溶剂中，50:50的辛醇:异丙醇显示出最佳结果，准确度为115%，重现性优于30%。
研究表明，透皮途径可用于猪皮肤中维生素D的分析。使用微针时，1小时内可获得准确测量结果。对于完整皮肤，提取2小时时准确度最高。
