Lawson D E, Douglas J, Lean M, Sedrani S
Clin Chim Acta. 1986 Jun 15;157(2):175-81. doi: 10.1016/0009-8981(86)90223-8.
An assay is described based on a high pressure liquid chromatography system for measurement of vitamin D3 and 25-hydroxyvitamin D3 concentration in adipose tissue and muscle. The sensitivity of the assay is less than 1 ng/g of tissue. Neither vitamin D3 nor 25-hydroxyvitamin D3 were found in tissues of vitamin D-deficient rats using this method. 25-Hydroxyvitamin D3 could not be detected in adipose tissue and very little was found in muscle of normal rats. The mean vitamin D3 concentrations in 15 samples of human perirenal adipose tissue was 45.3 ng/g +/- 22.2 (SD) and in 6 samples of axillary tissue 115.6 ng/g +/- 52.4 (SD). Human adipose tissue contains a substantial amount of vitamin D3 and its contribution to the maintenance of vitamin D status is discussed.
本文描述了一种基于高压液相色谱系统的检测方法，用于测量脂肪组织和肌肉中维生素D3和25-羟基维生素D3的浓度。该检测方法的灵敏度低于1 ng/g组织。使用此方法在维生素D缺乏大鼠的组织中未发现维生素D3和25-羟基维生素D3。在正常大鼠的脂肪组织中未检测到25-羟基维生素D3，在肌肉中发现的量很少。15份人肾周脂肪组织样本中维生素D3的平均浓度为45.3 ng/g +/- 22.2（标准差），6份腋窝组织样本中为115.6 ng/g +/- 52.4（标准差）。人脂肪组织含有大量维生素D3，并讨论了其对维持维生素D状态的作用。
