Super Ultra Low Energy and Emission Vehicle (SULEEV) Center , Korea University , Anam-ro 145 , Seongbuk-gu , Seoul 02841 , Republic of Korea.
Department of Chemical Engineering, Division of Chemical and Biological Engineering , Kangwon National University , Gangwon-Do 24341 , Republic of Korea.
Biomacromolecules. 2018 Dec 10;19(12):4534-4541. doi: 10.1021/acs.biomac.8b01221. Epub 2018 Nov 26.
Peptides are suitable candidates for templates in the fabrication of various metal nanoparticles (NPs) because of their metal-binding ability and templating effect, which impart physicochemical properties to the produced nanoparticles. Peptide-binding gold nanoparticles (AuNPs) show high catalytic activity that permits their application in oxidation or reduction reactions. Herein, we prepared morphology-controllable AuNPs stabilized by self-assembled tyrosine-rich peptides (YC) by varying the pH and YC peptide/Au concentration ratio in 2-( N-morpholino)ethanesulfonic acid (MES) buffer solution. The catalytic activities of the YC peptide-stabilized AuNPs (YC@AuNPs) were tested for 4-nitrophenol (4-NP) reduction, and kinetic analysis was performed to calculate the apparent rate constants and activation energies. The relatively low activation energy of the YC@AuNPs could be explained by the hypothesis that the tyrosine-moiety of YC enriches the electron density of Au metal.
肽是制造各种金属纳米粒子（NPs）的模板的合适候选物，因为它们具有金属结合能力和模板效应，赋予了所产生的纳米粒子的物理化学性质。肽结合的金纳米粒子（AuNPs）表现出高催化活性，允许它们在氧化或还原反应中应用。在此，我们通过在 2-（N-吗啉基）乙磺酸（MES）缓冲溶液中改变 pH 值和 YC 肽/ Au 浓度比，由自组装富含酪氨酸的肽（YC）制备了形态可控的 AuNPs。测试了 YC 肽稳定的 AuNPs（YC@AuNPs）对 4-硝基苯酚（4-NP）的还原的催化活性，并进行了动力学分析以计算表观速率常数和活化能。YC@AuNPs 的相对较低的活化能可以用假设来解释，即 YC 的酪氨酸部分增加了 Au 金属的电子密度。
