Department of Oral and Craniofacial Sciences, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
FEMS Yeast Res. 2019 Mar 1;19(2). doi: 10.1093/femsyr/foy123.
Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 hyphal-wall protein 1 (HWP1) are involved in hyphae formation and pathogenesis. The transcriptional agglutinin-like sequence 3 (ALS3) genes in both species are responsible for the development of biofilm and colonization on tooth surfaces. Therefore, we investigated the expression of HWP1 and ALS3 quantitatively in C. albicans and C. parapsilosis and examined the biofilm structure upon exposure to various nicotine concentrations. In vitro, biofilms of Candida species were developed directly on slides using the Lab-Tek Chamber Slide System and visualized by confocal laser scanning microscopy. Quantitative real-time polymerase chain reaction was used to measure HWP1 and ALS3 expression in C. albicans ATCC 14053 and C. parapsilosis ATCC 22019. The results indicated that nicotine multiplied the number of yeast cells and increased the extracellular polysaccharides of Candida species. We also found that 1-2 mg/mL nicotine could enhance the formation of biofilm. The findings also revealed that the expression of HWP1 and ALS3 in Candida species were increased as the nicotine concentration increased. Therefore, nicotine influences the biofilm development of oral-associated C. albicans ATCC 14053 and C. parapsilosis ATCC 22019.
白色念珠菌 ATCC14053 和近平滑念珠菌 ATCC22019 的菌丝壁蛋白 1（HWP1）参与菌丝形成和发病机制。这两个物种的转录凝集素样序列 3（ALS3）基因负责生物膜的发育和在牙齿表面的定植。因此，我们定量研究了白色念珠菌和近平滑念珠菌中 HWP1 和 ALS3 的表达，并检查了暴露于不同尼古丁浓度下生物膜的结构。在体外，使用 Lab-Tek 室玻片系统直接在载玻片上培养 Candida 物种的生物膜，并通过共聚焦激光扫描显微镜进行可视化。定量实时聚合酶链反应用于测量白色念珠菌 ATCC14053 和近平滑念珠菌 ATCC22019 中 HWP1 和 ALS3 的表达。结果表明，尼古丁使酵母细胞数量倍增，并增加了 Candida 物种的细胞外多糖。我们还发现 1-2mg/mL 的尼古丁可以增强生物膜的形成。研究结果还表明，随着尼古丁浓度的增加，白色念珠菌和近平滑念珠菌中 HWP1 和 ALS3 的表达增加。因此，尼古丁影响口腔相关的白色念珠菌 ATCC14053 和近平滑念珠菌 ATCC22019 的生物膜发育。
