Hoebee B, Brouwer J, van de Putte P, Loman H, Retèl J
Department of Biophysics, Free University, Amsterdam, The Netherlands.
Nucleic Acids Res. 1988 Aug 25;16(16):8147-56. doi: 10.1093/nar/16.16.8147.
Upon irradiation with gamma rays of an oxygenated aqueous solution of double-stranded M13 DNA, a very specific mutation spectrum was found with respect to both the type and the positions in the DNA sequence. Of the 23 mutations, which were sequenced, 16 represent a C/G to G/C transversion. A C/G to T/A transition was found once and a G/C to T/A transversion twice. The remaining 4 mutations are frameshifts, 2 are identical and formed by the insertion of a G/C basepair; the other 2 mutations are due to a duplication of 10 basepairs situated at different positions but with a remarkable homology in base sequence. Fourteen mutations, including the 2 duplications are found in the neighbourhood of a TGCT/ACGA sequence.
用γ射线辐照双链M13 DNA的含氧水溶液时，发现DNA序列中的突变类型和位置具有非常特定的突变谱。在已测序的23个突变中，16个代表C/G到G/C的颠换。发现一次C/G到T/A的转换和两次G/C到T/A的颠换。其余4个突变是移码突变，其中2个相同，由插入一个G/C碱基对形成；另外2个突变是由于位于不同位置但碱基序列具有显著同源性的10个碱基对的重复。包括这2个重复在内的14个突变出现在TGCT/ACGA序列附近。
