School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China.
School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Mar 5;210:348-354. doi: 10.1016/j.saa.2018.11.028. Epub 2018 Nov 13.
Fluorescence quenching was used to elucidate the binding interaction mechanism between bovine serum albumin (BSA) and graphene oxide (GO). By analyzing the values of Stern-Volmer quenching constant (K) and binding constant (K) which were affected by temperature, we supposed that the quenching process between GO and BSA was mainly determined by static quenching, combined with dynamic quenching. The study of thermodynamics showed that the values of enthalpy change (∆H), entropy change (∆S) and Free Energy (∆G) were all negative, which implied that the weak interaction of the molecular between BSA and GO was Van der Waals interaction or hydrogen bond, and the quenching process was exothermic and spontaneous. The red shift in the synchronous fluorescence spectra suggested that the conformation of tryptophan was changed in the presence of GO. According to Förster's non-radiative energy transfer theory, the distance r between BSA (donor) and GO (acceptor) was calculated and indicated the occurrence of energy transfer from BSA to GO had high probability. The AFM observation and Raman spectroscopy revealed that the interaction between BSA and GO has occurred. Compared with other literatures, the explosion of surface topography about BSA and GO was paid more attention on in this study.
荧光猝灭法被用来阐明牛血清白蛋白（BSA）与氧化石墨烯（GO）之间的结合相互作用机制。通过分析受温度影响的 Stern-Volmer 猝灭常数（K）和结合常数（K）的值，我们推测 GO 和 BSA 之间的猝灭过程主要由静态猝灭决定，同时伴有动态猝灭。热力学研究表明，焓变（∆H）、熵变（∆S）和自由能（∆G）的值均为负值，这意味着 BSA 和 GO 之间分子的弱相互作用是范德华力或氢键，猝灭过程是放热和自发的。同步荧光光谱的红移表明色氨酸的构象在存在 GO 的情况下发生了变化。根据福斯特的非辐射能量转移理论，计算了 BSA（供体）和 GO（受体）之间的距离 r，并表明 BSA 到 GO 的能量转移很可能发生。原子力显微镜观察和拉曼光谱表明，BSA 和 GO 之间发生了相互作用。与其他文献相比，本研究更关注 BSA 和 GO 之间表面形貌的爆炸。
