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利用氧化石墨烯及其衍生物改进人血清白蛋白的分离：一种用于临床应用的新方法。

Advancing Albumin Isolation from Human Serum with Graphene Oxide and Derivatives: A Novel Approach for Clinical Applications.

作者信息

Apiwat Chayachon, Houghton Jack W, Ren Ren, Tate Edward, Edel Joshua B, Chanlek Narong, Luksirikul Patraporn, Japrung Deanpen

机构信息

Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Thailand Science Park, Pathumthani 10120, Thailand.

出版信息

ACS Omega. 2024 Sep 20;9(39):40592-40607. doi: 10.1021/acsomega.4c04276. eCollection 2024 Oct 1.

DOI:10.1021/acsomega.4c04276
PMID:39371982
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11447712/
Abstract

This study introduces a novel, environmentally friendly albumin isolation method using graphene oxide (GO). GO selectively extracts albumin from serum samples, leveraging the unique interactions between GO's oxygen-containing functional groups and serum proteins. This method achieves high purification efficiency without the need for hazardous chemicals. Comprehensive characterization of GO and reduced graphene oxide (rGO) through techniques such as X-ray diffraction (XRD) analysis, Raman spectroscopy, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) confirmed the structural and functional group transformations crucial for protein binding. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry analyses demonstrated over 95% purity of isolated albumin, with minimal contamination from other serum proteins. The developed method, optimized for pH and incubation conditions, showcases a green, cost-effective, and simple alternative for albumin purification, promising broad applicability in biomedical research and clinical applications.

摘要

本研究介绍了一种使用氧化石墨烯（GO）的新型环保白蛋白分离方法。GO利用其含氧官能团与血清蛋白之间的独特相互作用，从血清样本中选择性提取白蛋白。该方法无需使用有害化学物质即可实现高纯化效率。通过X射线衍射（XRD）分析、拉曼光谱、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）等技术对GO和还原氧化石墨烯（rGO）进行的综合表征，证实了对蛋白质结合至关重要的结构和官能团转变。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和质谱分析表明，分离出的白蛋白纯度超过95%，其他血清蛋白的污染极小。针对pH值和孵育条件进行优化的所开发方法，展示了一种用于白蛋白纯化的绿色、经济高效且简单的替代方法，有望在生物医学研究和临床应用中得到广泛应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/12d3/11447712/8208cb9a0d30/ao4c04276_0011.jpg
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Nat Commun. 2023 Oct 17;14(1):6567. doi: 10.1038/s41467-023-42399-9.
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Human Serum Albumin Based Nanodrug Delivery Systems: Recent Advances and Future Perspective.基于人血清白蛋白的纳米药物递送系统：最新进展与未来展望
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Biochemical markers of nephrotic syndrome: An observational, cross-sectional study.肾病综合征的生化标志物：一项观察性横断面研究。
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Linking graphene-based material physicochemical properties with molecular adsorption, structure and cell fate.将基于石墨烯的材料的物理化学性质与分子吸附、结构及细胞命运联系起来。
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