瘦素通过 MAPK1/3 和 PI3K 信号通路刺激人牙髓中 DMP-1 和 DSPP 的表达。
Leptin stimulates DMP-1 and DSPP expression in human dental pulp via MAPK 1/3 and PI3K signaling pathways.
机构信息
Department of Stomatology (Endodontics section), School of Dentistry, University of Sevilla, C/ Avicena s/n, 41009, Sevilla, Spain.
Department of Medical Biochemistry and Molecular Biology, and Immunology, Vírgen Macarena University Hospital, University of Sevilla, Av. Dr. Fedriani 3, 41071, Sevilla, Spain.
出版信息
Arch Oral Biol. 2019 Feb;98:126-131. doi: 10.1016/j.archoralbio.2018.11.019. Epub 2018 Nov 20.
INTRODUCTION
To investigate the physiological function of leptin in human dental pulp, and to determine the specific pathways implicated in its effect.
METHODS
Twenty-seven dental pulp samples were obtained from human third molars. Pulp samples were treated with or without human recombinant leptin. Leptin functional effect was analyzed in terms of regulation of the synthesis levels of DSPP and DMP-1, determined by immunoblot.
RESULTS
Leptin stimulated DMP-1 and DSPP synthesis in all human dental pulp specimens. The stimulatory effect of leptin on DMP-1 and DSPP synthesis was partially prevented by blocking mitogen-activated protein kinase (MAPK 1/3) and phosphatidylinositol 3 kinase (PI3K) pathways, respectively.
CONCLUSIONS
The present study demonstrates the functional effect of leptin in human dental pulp stimulating the expression of DMP-1 and DSPP, both proteins implicated in dentinogenesis. Leptin stimulates DSPP expression via PI3K pathway and DMP-1 synthesis via MAPK 1/3 pathway. These results support the role of leptin in pulpal reparative response, opening a new research line that could have translational application to the clinic in vital pulp therapy procedures.
简介
研究瘦素在人牙髓中的生理功能，并确定其作用所涉及的具体途径。
方法
从人类第三磨牙中获得 27 个人牙髓样本。用或不用人重组瘦素处理牙髓样本。通过免疫印迹分析瘦素对 DSPP 和 DMP-1 合成水平的调节作用来分析瘦素的功能效应。
结果
瘦素刺激所有人类牙髓标本中 DMP-1 和 DSPP 的合成。瘦素对 DMP-1 和 DSPP 合成的刺激作用分别通过阻断丝裂原活化蛋白激酶（MAPK1/3）和磷脂酰肌醇 3 激酶（PI3K）途径而部分阻断。
结论
本研究表明瘦素在人牙髓中的功能作用刺激 DMP-1 和 DSPP 的表达，这两种蛋白质都与牙本质形成有关。瘦素通过 PI3K 途径刺激 DSPP 表达，通过 MAPK1/3 途径刺激 DMP-1 合成。这些结果支持瘦素在牙髓修复反应中的作用，为临床在活髓治疗过程中的应用开辟了一条新的研究途径。
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