College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Biomedical Sciences , Shandong Normal University , Jinan 250014 , People's Republic of China.
Anal Chem. 2018 Dec 18;90(24):14507-14513. doi: 10.1021/acs.analchem.8b04354. Epub 2018 Dec 6.
Effective and sensitive monitoring of arsenate in drinking water is significant for risk management of public health. Here, we demonstrated that a CeO nanowire acted as an efficient quencher for small fluorescent molecules with a phosphate group, BODIPY-adenosine triphosphate (BODIPY-ATP) and riboflavin-5'-phosphate (Rf-P), and developed a CeO nanowire-BODIPY-ATP platform for highly selective and sensitive detection of arsenate. The response strategy was based on the competitive coordination chemistry of CeO nanowire between arsenate and phosphate group of BODIPY-ATP. Arsenate displaced adsorbed BODIPY-ATP to enhance fluorescence, allowing detection of arsenate down to 7.8 nM, which is lower than the WHO-defined limit of 130 nM. An excellent linear range of 20-150 and 150-1000 nM was obtained. Importantly, this system was simple in design and convenient in operation. Also, the platform exhibited excellent selectivity for arsenate without the interference of phosphate ions. Finally, the proposed method had been successfully employed for determination of arsenate in real water samples.
有效且灵敏地监测饮用水中的砷酸盐对于公共卫生风险管理具有重要意义。在这里，我们证明了 CeO 纳米线可作为带磷酸基团的小分子荧光分子（BODIPY-三磷酸腺苷（BODIPY-ATP）和核黄素-5'-磷酸（Rf-P）的有效猝灭剂，并开发了 CeO 纳米线-BODIPY-ATP 平台，用于高选择性和灵敏地检测砷酸盐。该响应策略基于 CeO 纳米线与 BODIPY-ATP 的磷酸基团之间的竞争配位化学。砷酸盐取代吸附的 BODIPY-ATP 以增强荧光，可检测到低至 7.8 nM 的砷酸盐，低于世界卫生组织定义的 130 nM 限值。获得了 20-150 和 150-1000 nM 的优异线性范围。重要的是，该系统设计简单，操作方便。此外，该平台对砷酸盐表现出优异的选择性，不受磷酸根离子的干扰。最后，该方法已成功用于实际水样中砷酸盐的测定。
