College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China.
College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China.
Talanta. 2018 Mar 1;179:285-291. doi: 10.1016/j.talanta.2017.09.091. Epub 2017 Oct 2.
A simple, rapid, and ultrasensitive fluorescence strategy for adenosine triphosphate (ATP) detection was developed by using a FAM (carboxyfluorescein) labeled DNA (FAM-DNA). In this strategy, highly fluorescent FAM-DNA was used as a probe, and nanoceria (CeO NPs) acted as an efficient quencher. FAM-DNA attached to the surface of nanoceria through the coordination between the phosphate group of DNA and NP surface, which induced complete quenching in the FAM-DNA fluorescence due to a photo induced electron transfer (PET) process. It was found that ATP can readily displace adsorbed DNA from nanoceria surface because of the stronger coordination ability of ATP with nanoceria, and the nanoceria-based competitive binding resulted in over 7-fold fluorescence enhancement. Over a wide range from 0.1nM to 1.5μM, a good linear relationship between the fluorescence intensity and the concentration of ATP was obtained and the detection limit was estimated to be as low as 54pM. This method was successfully used to analyze ATP in a single drop of blood and human urine.
一种简单、快速、超灵敏的荧光策略用于检测三磷酸腺苷（ATP），该策略使用了羧基荧光素（FAM）标记的 DNA（FAM-DNA）。在该策略中，高荧光 FAM-DNA 被用作探针，纳米氧化铈（CeO NPs）作为有效的猝灭剂。FAM-DNA 通过 DNA 磷酸基团与 NP 表面的配位附着在纳米氧化铈表面，由于光诱导电子转移（PET）过程，导致 FAM-DNA 荧光完全猝灭。研究发现，由于 ATP 与纳米氧化铈的配位能力更强，ATP 可以轻易地从纳米氧化铈表面置换出吸附的 DNA，基于纳米氧化铈的竞争结合导致荧光增强超过 7 倍。在 0.1nM 至 1.5μM 的较宽范围内，荧光强度与 ATP 浓度之间呈现良好的线性关系，检测限估计低至 54pM。该方法成功地用于分析一滴血液和人尿中的 ATP。
