Wilson Daisy, Breen Leigh, Lord Janet M, Sapey Elizabeth
MRC-ARUK Centre for Musculoskeletal Ageing Research, Institute of Inflammation and Ageing, University of Birmingham, Queen Elizabeth Hospital Birmingham, Edgbaston, Birmingham, B15 2WD, UK.
BMC Res Notes. 2018 Nov 26;11(1):830. doi: 10.1186/s13104-018-3947-8.
To describe the difficulties of obtaining muscle samples using a Bergstrom needle technique in a frail older adult population. The data were obtained from a study primarily investigating immunosenescence in frailty. An intended research technique was skeletal muscle biopsy in a small subset of participants to investigate muscle morphology and local inflammatory factors.
Forty healthy older adults and 37 frail older adults were considered for a Bergstrom needle muscle biopsy. Of these, 17.5% of healthy older adults and 94.6% of the frail older adults had single or multiple participant factors resulting in a contra-indication to muscle biopsy. 40.7% of healthy older female participants were at risk of a failed muscle biopsy due to low muscle mass. Considering only muscle mass muscle biopsy would have been successful in 18.7% of the frail older women and 21.4% of the frail older men. In this population, muscle biopsy was not feasible because of contra-indications in the majority of participants. This questions whether a biopsy sample obtained from frail older individuals, is actually representative of this population and supports the need to disclose biopsy failure rate in this population.
描述在体弱的老年人群中使用贝格斯特龙针技术获取肌肉样本的困难。数据来自一项主要研究体弱状态下免疫衰老的研究。一项预期的研究技术是对一小部分参与者进行骨骼肌活检，以研究肌肉形态和局部炎症因子。
40名健康老年人和37名体弱老年人被考虑进行贝格斯特龙针肌肉活检。其中，17.5%的健康老年人和94.6%的体弱老年人存在单一或多个参与者因素，导致肌肉活检存在禁忌证。40.7%的健康老年女性参与者因肌肉量低而有肌肉活检失败的风险。仅考虑肌肉量，肌肉活检在18.7%的体弱老年女性和21.4%的体弱老年男性中可能成功。在该人群中，由于大多数参与者存在禁忌证，肌肉活检不可行。这就引发了从体弱老年人身上获取的活检样本是否真的能代表该人群的疑问，并支持在该人群中披露活检失败率的必要性。
