Iwai Maki, Harada Yuichirou, Miyabayashi Rinako, Kang Woojin, Nakamura Akihiro, Kawano Natsuko, Miyamoto Yoshitaka, Yamada Mitsutoshi, Hamatani Toshio, Miyado Mami, Yoshida Keiichi, Saito Hidekazu, Tanaka Mamoru, Umezawa Akihiro, Miyado Kenji
Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo 160-8582, Japan.
Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan.
Heliyon. 2018 Nov 16;4(11):e00944. doi: 10.1016/j.heliyon.2018.e00944. eCollection 2018 Nov.
Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca storage and were colocalized with endoplasmic reticulum (ER) during Ca oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation.
线粒体是真核细胞的动力源，其定位有助于受精和早期发育过程。我们报告称，精子融合会触发小鼠卵子中的钙振荡以及线粒体向融合精子的移动（线粒体趋化性）。线粒体在钙储存中发挥作用，并且在钙振荡期间与内质网（ER）共定位。然后线粒体向融合精子移动。缺乏细胞核的精子提取物可诱导钙振荡，但不会促进线粒体趋化性。我们的结果表明，精子融合会激发不依赖钙振荡的线粒体趋化性。这一现象表明，卵子线粒体与精子物质（可能是核物质）相互作用，并且它们的网络在合子形成的早期阶段将卵子和精子细胞核连接在一起。
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