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作者信息

Grabova A Yu, Dragovoz I V, Zelena L B, Ostapchuk A N, Avdeeva L V

出版信息

Tsitol Genet. 2016 Jul-Aug;50(4):62-8.

Abstract

The polyphasic taxonomic analysis of the strain-antagonist of phytopathogenic bacteria and micromycetes was carried. It was found that the combination of culture-morphological, physiological and biochemical properties allowed to attribute the strain to the group Bacillus subtilis. It was shown that fatty acids of the cell walls of the strain were represented mainly by branched derivatives of iso- and anti iso- C15:0 and C17:0 fatty acids (85%) which was typical for the Bacillus amyloliquefaciens species. After molecular genetic analysis of the nucleotide sequence of the 16S rRNA gene and studying the profile of polymorphic nucleotides the strain was attributed to subspecies Bacillus amyloliquefaciens subsp. plantarum.

摘要

对植物病原菌和微真菌的拮抗菌株进行了多相分类分析。结果发现,培养形态学、生理生化特性的组合使该菌株归为枯草芽孢杆菌属。结果表明,该菌株细胞壁脂肪酸主要由异-C15:0和反异-C17:0脂肪酸的支链衍生物组成(85%),这是解淀粉芽孢杆菌种的典型特征。对16S rRNA基因核苷酸序列进行分子遗传分析并研究多态性核苷酸谱后,该菌株被归为解淀粉芽孢杆菌植物亚种。

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