State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering , Harbin Institute of Technology , 92 West Da-Zhi Street , Harbin 150001 , China.
Interdisciplinary Nanoscience Center (iNANO) , Aarhus University , Gustav Wieds Vej 14 , 8000 Aarhus , Denmark.
Anal Chem. 2018 Dec 18;90(24):14363-14367. doi: 10.1021/acs.analchem.8b03825. Epub 2018 Nov 27.
Giant unilamellar vesicles (GUVs) are widely used as artificial cell models which contribute to elucidate fundamental questions on origin of life and cell functions. Herein, the GUV microarrays were developed using a point-to-plane electrode system combined with microcontact stripping technique. The biomolecules (DNA, etc.) were selectively encapsulated only inside patterned GUVs. The GUV arrays were used to investigate species mass transport across cell membranes. The release of carboxyfluorescein from GUVs showed a melittin concentration dependent manner. The diffusion coefficient were 0.37 × 10, 0.36 × 10, 0.54 × 10, 1.10 × 10, 1.74 × 10, 2.31 × 10, and 3.62 × 10 m/s for 0, 0.5, 1.0, 2.0, 3.0, 4.0, and 5.0 μM melittin, respectively. The GUV arrays were also a good platform for cell metabolism investigation. Horseradish peroxidase (HRP) loaded GUV microarrays were used to mimic internal metabolism by exposing them to the substrates of HO and o-PD to yield fluorescent 2,3-diaminophenazine (2,3-DAP).The proposed GUV arrays have great potential in cell function studies.
巨大的单室囊泡 (GUV) 被广泛用作人工细胞模型，有助于阐明生命起源和细胞功能的基本问题。在此，使用点对面电极系统结合微接触剥离技术开发了 GUV 微阵列。生物分子（DNA 等）仅选择性地封装在图案化的 GUV 内。GUV 阵列用于研究跨细胞膜的物种质量传输。从 GUV 释放羧基荧光素呈蜂毒素浓度依赖性。扩散系数分别为 0.37×10、0.36×10、0.54×10、1.10×10、1.74×10、2.31×10 和 3.62×10 m/s，用于 0、0.5、1.0、2.0、3.0、4.0 和 5.0 μM 蜂毒素。GUV 阵列也是细胞代谢研究的良好平台。辣根过氧化物酶 (HRP) 负载的 GUV 微阵列用于通过将其暴露于 HO 和 o-PD 的底物中来模拟内部代谢，以产生荧光 2,3-二氨基吩嗪 (2,3-DAP)。所提出的 GUV 阵列在细胞功能研究中具有巨大的潜力。
