米康唑负载壳聚糖纳米粒局部治疗外阴阴道假丝酵母菌病真菌感染。
Miconazole loaded chitosan-based nanoparticles for local treatment of vulvovaginal candidiasis fungal infections.
机构信息
Biotechnology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, 74605-050, Brazil.
VIB-UGent Center for Inflammation Research, Ghent 9052, Belgium.
出版信息
Colloids Surf B Biointerfaces. 2019 Feb 1;174:409-415. doi: 10.1016/j.colsurfb.2018.11.048. Epub 2018 Nov 20.
OBJECTIVES
In this study, polymeric nanoparticles based on chitosan incorporating the antifungal miconazole nitrate were fabricated and testedin vivo using murine vulvovaginal candidiasis.
METHODS
Nanoparticles prepared by the ionotropic gelation method presented 200 to 300 nm diameter and polydispersity indexes ranging from 0.2 to 0.4. The nanoparticles were prepared to incorporate 63.9 mg/mL of miconazole nitrate to be testedin vivo. Murine vulvovaginal candidiasis was standardized using estradiol valerate before the animals were challenged by Candida albicans.
RESULTS
The treatment using chitosan nanoparticles within miconazole nitrate presented the same therapeutic efficacy as miconazole nitrate in a commercial cream formulation, however using the antifungal content about seven-fold lower. This increase in the miconazole nitrate's therapeutic efficacy is may be due to the down-regulation of interleukin 10 (IL-10) expression.
CONCLUSIONS
Our data represent a proof of concept that can be exploited to achieve an alternative and promising therapy for the treatment of vulvovaginal candidiasis.
目的
本研究采用离子凝胶法制备了载有抗真菌药物硝酸咪康唑的壳聚糖纳米粒子，并通过小鼠阴道假丝酵母菌病模型进行了体内试验。
方法
所制备的纳米粒子粒径为 200 至 300nm，多分散指数为 0.2 至 0.4。纳米粒子的载药量为 63.9mg/mL 的硝酸咪康唑，用于体内试验。在动物受到白色念珠菌攻击之前，使用戊酸雌二醇使小鼠阴道假丝酵母菌病标准化。
结果
与市售乳膏制剂中的硝酸咪康唑相比，使用壳聚糖纳米粒子载药的治疗方法具有相同的疗效，但所用的抗真菌药物含量低约 7 倍。这种硝酸咪康唑治疗效果的提高可能是由于白细胞介素 10（IL-10）表达下调所致。
结论
我们的数据提供了一个概念验证，可以用于开发治疗阴道假丝酵母菌病的替代且有前景的治疗方法。
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