Pharmaco-Toxicological Analysis Laboratory (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Bologna, Italy.
Department for Life Quality Studies, Alma Mater Studiorum - University of Bologna, Rimini, Italy.
Anal Chim Acta. 2019 Jan 10;1046:32-47. doi: 10.1016/j.aca.2018.09.004. Epub 2018 Sep 5.
Volumetric absorptive microsampling (VAMS) is a recent microsampling technique used to obtain dried specimens of blood and other biological matrices for application to a plethora of bioanalytical purposes. As such, it can be likened to dried blood spot (DBS) technique that has been in wide use for the last 40 years. However, VAMS promises to bring some significant advantages over DBS, related to sampling volume accuracy, haematocrit (HCT) dependence, pre-treatment and automation. Although some aspects still need to be investigated in depth, VAMS is increasingly recognised as a viable alternative to DBS and other dried microsampling techniques. In this tutorial, different aspects of VAMS approach are described and discussed, presenting the procedures adopted and the results obtained by those authors who have developed this kind of analytical workflow in the last few years. Hopefully, this will help other scientists to find new solutions to old and recent problems related to microsampling and to produce new, sound and interesting science in this field.
体积吸收微采样（VAMS）是一种最近的微采样技术，用于获取血液和其他生物基质的干燥样本，以应用于大量的生物分析目的。因此，它可以与过去 40 年来广泛使用的干血斑（DBS）技术相媲美。然而，VAMS 有望在采样体积准确性、血细胞比容（HCT）依赖性、预处理和自动化方面带来一些显著优势。尽管仍有一些方面需要深入研究，但 VAMS 越来越被认为是 DBS 和其他干燥微采样技术的可行替代品。在本教程中，描述和讨论了 VAMS 方法的不同方面，介绍了过去几年开发这种分析工作流程的作者所采用的程序和获得的结果。希望这将帮助其他科学家找到解决与微采样相关的旧问题和新问题的新解决方案，并在该领域产生新的、合理的和有趣的科学。
