The antibacterial effect of aquatic and methanolic extract of on , and .

BACKGROUND AND OBJECTIVES

Anaerobic Gram negative bacteria are the main cause of periodontal destruction. It has been shown that have anti-bacterial activity on Gram positive and Gram negative bacteria. The aim of this study was to determine the antibacterial effect of aquatic and methanolic extract of on some of the oral Gram-negative bacteria.

MATERIALS AND METHODS

The antibacterial effect of aquatic and methanolic extracts of was determined using disk diffusion method at different concentrations from 10 to 500 mg/ml. The diameter of inhibition zones were determined. The MIC was defined using the standard broth macrodilution method. The results of the study were reported descriptively.

RESULTS

The aquatic extract of from 20 to 500 mg/ml had antibacterial effect on and The methanolic extract from 10 to 500 mg/ml had antibacterial effect on and . The MIC was achieved at 10 mg/ml, 10 mg/ml and 10 mg/ml for aquatic and methanolic extracts of on and , respectively.

CONCLUSION

Aquatic and methanolic extracts of had antibacterial effect on and . Most concentrations of aqueous extract were effective on bacteria, so, providing an alcoholic extract, that is a time consuming and costly method, does not seem necessary.