Rahimvand Leila, Niakan Mohammad, Naderi Noushin Jalayer
Faculty of Dentistry, Shahed University, Tehran, Iran.
Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.
Iran J Microbiol. 2018 Aug;10(4):254-257.
Anaerobic Gram negative bacteria are the main cause of periodontal destruction. It has been shown that have anti-bacterial activity on Gram positive and Gram negative bacteria. The aim of this study was to determine the antibacterial effect of aquatic and methanolic extract of on some of the oral Gram-negative bacteria.
The antibacterial effect of aquatic and methanolic extracts of was determined using disk diffusion method at different concentrations from 10 to 500 mg/ml. The diameter of inhibition zones were determined. The MIC was defined using the standard broth macrodilution method. The results of the study were reported descriptively.
The aquatic extract of from 20 to 500 mg/ml had antibacterial effect on and The methanolic extract from 10 to 500 mg/ml had antibacterial effect on and . The MIC was achieved at 10 mg/ml, 10 mg/ml and 10 mg/ml for aquatic and methanolic extracts of on and , respectively.
Aquatic and methanolic extracts of had antibacterial effect on and . Most concentrations of aqueous extract were effective on bacteria, so, providing an alcoholic extract, that is a time consuming and costly method, does not seem necessary.
厌氧革兰氏阴性菌是牙周组织破坏的主要原因。已有研究表明，[具体物质]对革兰氏阳性菌和革兰氏阴性菌均具有抗菌活性。本研究旨在确定[具体物质]的水提取物和甲醇提取物对某些口腔革兰氏阴性菌的抗菌效果。
采用纸片扩散法，在10至500毫克/毫升的不同浓度下，测定[具体物质]水提取物和甲醇提取物的抗菌效果。测量抑菌圈直径。采用标准肉汤稀释法确定最低抑菌浓度（MIC）。对研究结果进行描述性报告。
20至500毫克/毫升的[具体物质]水提取物对[具体细菌1]和[具体细菌2]具有抗菌作用。10至500毫克/毫升的甲醇提取物对[具体细菌3]和[具体细菌4]具有抗菌作用。[具体物质]水提取物和甲醇提取物对[具体细菌1]、[具体细菌3]和[具体细菌4]的MIC分别为10毫克/毫升、10毫克/毫升和10毫克/毫升。
[具体物质]的水提取物和甲醇提取物对[具体细菌1]、[具体细菌2]、[具体细菌3]和[具体细菌4]具有抗菌作用。大多数浓度的水提取物对这些细菌均有效，因此，制备耗时且成本高的醇提取物似乎没有必要。
