Ghasemi Sepideh, Aligholi Hadi, Koulivand Pir Hossein, Jafarian Maryam, Hosseini Ravandi Hassan, Khaleghi Ghadiri Maryam, Gorji Ali
Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran.
Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
Iran J Basic Med Sci. 2018 Nov;21(11):1155-1160. doi: 10.22038/IJBMS.2018.29587.7146.
Among several cell sources, adult human neural stem/progenitor cells (hNS/PCs) have been considered outstanding cells for performing mechanistic studies in in vitro and in vivo models of neurological disorders as well as for potential utility in cell-based therapeutic approaches. Previous studies addressed the isolation and culture of hNS/PCs from human neocortical and hippocampal tissues. However, little data are available on hNS/PCs obtained from the adult human amygdala.
The present study explored the capacity of the amygdala harvested from resected brain tissues of patients with medically refractory epilepsy to generate neurosphere-like bodies and motor neuron-like cells.
Although the proliferation process was slow, a considerable amount of cells was obtained after the 3rd passage. In addition, the cells could generate motor neuron-like cells under appropriate culture conditions.
Isolation and culture of these cells enable us to improve our knowledge of the role of the amygdala in some neurological and psychological disorders and provide a novel source for therapeutic cell transplantation.
在多种细胞来源中，成人人类神经干细胞/祖细胞（hNS/PCs）被认为是在神经疾病的体外和体内模型中进行机制研究以及在基于细胞的治疗方法中具有潜在效用的优秀细胞。先前的研究涉及从人类新皮质和海马组织中分离和培养hNS/PCs。然而，关于从成人人类杏仁核获得的hNS/PCs的数据很少。
本研究探讨了从药物难治性癫痫患者切除的脑组织中获取的杏仁核产生神经球样体和运动神经元样细胞的能力。
尽管增殖过程缓慢，但在第3代后获得了大量细胞。此外，这些细胞在适当的培养条件下可以产生运动神经元样细胞。
这些细胞的分离和培养使我们能够增进对杏仁核在某些神经和心理疾病中作用的了解，并为治疗性细胞移植提供新的来源。
