Picard Christine J, Wells Jeffrey D, Ullyot Anne, Rognes Knut
Department of Biology & Forensic and Investigative Sciences Program, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA.
Department of Biological Sciences & International Forensic Research Institute, Florida International University, Miami, FL, USA.
Forensic Sci Res. 2017 Dec 8;3(1):60-64. doi: 10.1080/20961790.2017.1398286. eCollection 2018.
Common DNA-based species determination methods fail to distinguish some blow flies in the forensically and medically important genus Robineau-Desvoidy. This is a practical problem, and it has also been interpreted as casting doubt on the validity of some morphologically defined species. An example is and , which co-occur in Europe whilst only has been collected in North America. Reports that these species shared both mitochondrial and nuclear gene sequences, along with claims that diagnostic morphological characters are difficult to interpret, were used to question their separate species status. We report here that amplified fragment length polymorphism profiles strongly support the validity of both species based on both assignment and phylogenetic analysis, and that traditional identification criteria based on male and female genital morphology are more reliable than has been claimed.
常见的基于DNA的物种鉴定方法无法区分法医和医学上重要的罗宾诺-德沃伊属中的一些丽蝇。这是一个实际问题，也有人将其解释为对一些形态学定义物种的有效性提出质疑。一个例子是 和 ，它们在欧洲共同出现，而在北美只采集到了 。有报道称这些物种共享线粒体和核基因序列，同时有人声称诊断性形态特征难以解释，这些都被用来质疑它们作为独立物种的地位。我们在此报告，基于扩增片段长度多态性图谱，通过归类和系统发育分析有力地支持了这两个物种的有效性，并且基于雄性和雌性生殖器形态的传统鉴定标准比人们声称的更可靠。
