Institute of Forensic Medicine, Goethe-University Frankfurt, Kennedyallee 104, 60596, Frankfurt am Main, Germany.
Parasitol Res. 2012 Jun;110(6):2325-32. doi: 10.1007/s00436-011-2767-8. Epub 2011 Dec 21.
Deoxyribonucleic acid (DNA)-based insect identification has become a routine and accurate tool in forensic entomology. In the present study, we demonstrate the utility of the mitochondrial DNA cytochrome oxidase I gene "barcoding region" as a universal marker for molecular identification of forensically important Diptera. We analyzed 111 specimens belonging to 13 species originating from Frankfurt am Main, Germany (Calliphoridae: Calliphora vicina, Calliphora vomitoria, Lucilia ampullacea, Lucilia caesar, Lucilia illustris, Lucilia sericata, Lucilia silvarum, Phormia regina, Protophormia terraenovae; Piophilidae: Parapiophila vulgaris; Muscidae: Hydrotaea dentipes, Hydrotaea ignava, Hydrotaea similis). Intraspecific variation ranged from 0 to 1.17% and interspecific variation occurred between 1.17% and 15.21%. Although differences within species were generally less than among species, divergence percentages overlapped due to low interspecific nucleotide divergence of the recently separated sister species L. caesar and L. illustris. However, all species formed distinct monophyletic clades and thus the cytochrome oxidase 1 (COI) barcode has been shown suitable for clear differentiation and identification of forensically relevant Diptera in Germany.
基于脱氧核糖核酸（DNA）的昆虫鉴定已成为法医昆虫学中一种常规且准确的工具。在本研究中，我们展示了线粒体 DNA 细胞色素氧化酶 I 基因“条形码区域”作为法医重要双翅目昆虫分子鉴定的通用标记的实用性。我们分析了 111 个标本，这些标本来自德国美因河畔法兰克福的 13 个物种（丽蝇科：Calliphora vicina、Calliphora vomitoria、Lucilia ampullacea、Lucilia caesar、Lucilia illustris、Lucilia sericata、Lucilia silvarum、Phormia regina、Protophormia terraenovae；皮蝇科：Parapiophila vulgaris；蝇科：Hydrotaea dentipes、Hydrotaea ignava、Hydrotaea similis）。种内变异范围为 0 至 1.17%，种间变异发生在 1.17%至 15.21%之间。尽管种内差异通常小于种间差异，但由于最近分离的姐妹种 L. caesar 和 L. illustris 之间的核苷酸差异较小，因此出现了差异百分比重叠。然而，所有物种都形成了独特的单系分支，因此细胞色素氧化酶 1（COI）条码已被证明适合于德国法医相关双翅目昆虫的清晰区分和鉴定。
