National Agronomic Institute of Tunisia (INAT), 43, Avenue Charles Nicolle, Mahrajène, 1082, Tunis, Tunisia.
Laboratory "Energy and Matter for Development of Nuclear Sciences" (LR16CNSTN02), National Center for Nuclear Sciences and Technology, Sidi Thabet Technopark, 2020, Sidi Thabet, Tunisia.
Environ Sci Pollut Res Int. 2020 Feb;27(6):5661-5669. doi: 10.1007/s11356-018-3709-0. Epub 2018 Nov 27.
The majority of dosimeters currently in use are synthetic and very expensive. Therefore, the study of the dosimetric characteristics of polyphenolic extracts of xerophytes is useful because drought stress causes an increase in the production of these cheap and natural compounds containing benzene rings. Here, the polyphenolic compounds were extracted from Rhamnus lycioides which was collected from Bou-Hedma National Park in Tunisia and identified using liquid chromatography-mass spectrometry (LC-MS). We investigated the impact of cobalt-60 (Co) irradiation (0-30 kilogray (kGy)) on the color parameters of polyphenolic extracts of R. lycioides using the Konica Minolta CR 300 portable colorimeter and UV-Visible spectroscopy. The structural and morphological characteristics of the irradiated extracts were assessed using Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) technique and scanning electron microscopy (SEM). Overall, our results suggest that exposure to ionizing radiation (IR) of the polyphenolic components of the xerophyte R. lycioides has produced significant dose-dependent changes in their optical and morphological properties. Thus, these extracts can be valorized as biodosimeters in the dose range from 5 to 25 kGy.
目前使用的大多数剂量计都是合成的，而且非常昂贵。因此，研究旱生植物多酚提取物的剂量学特性是有用的，因为干旱胁迫会导致这些廉价天然含苯环化合物的产量增加。在这里，从突尼斯 Bou-Hedma 国家公园采集的 Rhamnus lycioides 中提取了多酚化合物，并使用液相色谱-质谱联用仪 (LC-MS) 进行了鉴定。我们使用 Konica Minolta CR 300 便携式色度计和紫外可见分光光度计研究了钴-60（Co）辐照（0-30 千戈瑞（kGy））对 R. lycioides 多酚提取物颜色参数的影响。使用傅里叶变换红外（FT-IR）光谱、X 射线衍射（XRD）技术和扫描电子显微镜（SEM）评估了辐照提取物的结构和形态特征。总的来说，我们的结果表明，暴露于离子辐射（IR）会导致旱生植物 R. lycioides 中多酚成分的光学和形态特性发生显著的剂量依赖性变化。因此，这些提取物可以在 5 至 25 千戈瑞的剂量范围内作为生物剂量计进行评估。
