Hiroshima Synchrotron Radiation Center, Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan.
Adv Exp Med Biol. 2018;1104:101-117. doi: 10.1007/978-981-13-2158-0_6.
Circular-dichroism (CD) spectroscopy is a powerful tool for analyzing the structures of chiral molecules and biomolecules. The development of CD instruments using synchrotron radiation has greatly expanded the utility of this method by extending the spectra to the vacuum-ultraviolet (VUV) region below 190 nm and thereby yielding information that is unobtainable by conventional CD instruments. This technique is especially advantageous for monitoring the structure of saccharides that contain hydroxy and acetal groups with high-energy transitions in the VUV region. Combining VUVCD spectra with theoretical calculations provides new insight into the contributions of anomeric hydroxy groups and rotational isomers of hydroxymethyl groups to the dynamics, intramolecular hydrogen bonds, and hydration of saccharides in aqueous solution.
圆二色性（CD）光谱学是分析手性分子和生物分子结构的有力工具。利用同步辐射开发的 CD 仪器通过将光谱扩展到 190nm 以下的真空紫外（VUV）区域，极大地扩展了这种方法的用途，从而提供了常规 CD 仪器无法获得的信息。该技术特别有利于监测含有羟基和缩醛基团的糖的结构，因为这些基团在 VUV 区域具有高能跃迁。将 VUVCD 光谱与理论计算相结合，为糖在水溶液中的动力学、分子内氢键和水合作用中端基羟基和羟甲基旋转异构体的贡献提供了新的认识。
