Šimják P, Cinkajzlová A, Anderlová K, Kloučková J, Kratochvílová H, Lacinová Z, Kaválková P, Krejčí H, Mráz M, Pařízek A, Kršek M, Haluzík M
Gynecology and Obstetrics Department, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
Physiol Res. 2018 Nov 28;67(Suppl 3):S531-S542. doi: 10.33549/physiolres.934017.
We measured plasma concentrations, adipose tissue and placental mRNA expression of hepatokines fetuin A, fetuin B and fibroblast growth factor 21 (FGF21) in 12 healthy pregnant women (P group), 12 pregnant women with gestational diabetes (GDM) and 10 healthy non-pregnant women (N group) to explore their potential role in the etiopathogenesis of GDM. GDM and P group had comparable BMI, C-reactive protein (CRP) and glycated hemoglobin levels while IL-10 and TNF-alpha levels were higher in GDM group. Fetuin A and fetuin B levels were higher in pregnancy as compared to N group and decreased after delivery with no apparent influence of GDM. In contrast, the pattern of changes of circulating FGF21 levels differed between GDM and P group. Fetuin A concentrations positively correlated with CRP, TNF-alpha mRNA expression in adipose tissue and IL-6 mRNA expression in placenta. Fetuin B positively correlated with CRP. FGF21 levels correlated positively with IFN-gamma mRNA in adipose tissue and inversely with IL-8 mRNA in the placenta. Taken together, fetuin A and fetuin B levels were increased during pregnancy regardless of the presence of GDM. In contrast, FGF21 patterns differed between healthy pregnant women and GDM patients suggesting a possible role of this hepatokine in the etiopathogenesis of GDM.
我们检测了12名健康孕妇（P组）、12名妊娠期糖尿病孕妇（GDM组）和10名健康非孕妇（N组）血浆中胎球蛋白A、胎球蛋白B和成纤维细胞生长因子21（FGF21）的浓度，以及脂肪组织和胎盘的mRNA表达，以探讨它们在GDM发病机制中的潜在作用。GDM组和P组的BMI、C反应蛋白（CRP）和糖化血红蛋白水平相当，而GDM组的IL-10和TNF-α水平较高。与N组相比，胎球蛋白A和胎球蛋白B水平在孕期升高，产后下降，且不受GDM的明显影响。相比之下，GDM组和P组循环FGF21水平的变化模式不同。胎球蛋白A浓度与CRP、脂肪组织中TNF-α mRNA表达及胎盘中IL-6 mRNA表达呈正相关。胎球蛋白B与CRP呈正相关。FGF21水平与脂肪组织中IFN-γ mRNA呈正相关，与胎盘中IL-8 mRNA呈负相关。综上所述，无论是否存在GDM，胎球蛋白A和胎球蛋白B水平在孕期均升高。相比之下，健康孕妇和GDM患者的FGF21模式不同，提示这种肝因子在GDM发病机制中可能起作用。
