Khalife Esmat, Khodadadi Ali, Talaeizadeh Abdolhosein, Rahimian Leila, Nemati Maryam, Jafarzadeh Abdollah
Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Email:
Asian Pac J Cancer Prev. 2018 Nov 28;19(11):3019-3025. doi: 10.31557/APJCP.2018.19.11.3019.
Background: Regulatory T (Treg) cells are immunosuppressor lymphocytes that play a critical role in the establishment and progression of cancers. A number of markers, especially FOXP3, CTLA-4 and GITR influence the function of Treg cells. This investigation aimed to evaluate the expression of a number of important Treg cell-related markers by peripheral blood mononuclear cells (PBMCs) from newly-diagnosed women with breast cancer. Methods: The fresh PBMCs were obtained from 20 women with breast cancer and 20 healthy individuals. The PBMCs from both groups were cultured for 32 hours in the presence or absence of PHA (10 μg/ml). After total RNA extraction from cultured PBMCs, the expression of the FOXP3, CTLA-4 and GITR transcripts was assessed using real time-PCR. Results: The mRNA expression of FOXP3, CTLA-4 and GITR in unstimulated PBMCs from patients with breast cancer were significantly higher than healthy control group (P<0.05, P<0.03 and P<0.04, respectively). Similarly, the expression of FOXP3, CTLA-4 and GITR transcripts in PHA-stimulated PBMCs from patients with breast cancer were significantly increased in comparison with healthy individuals (P<0.01, P<0.005 and P<0.01, respectively). Conclusion: The increased expression of FOXP3, CTLA-4 and GITR represent higher activity of Treg cells in patients with breast cancer that may play an important role in the tumor establishment and development.
调节性T（Treg）细胞是免疫抑制淋巴细胞，在癌症的发生和发展中起关键作用。许多标志物，尤其是FOXP3、CTLA-4和糖皮质激素诱导的肿瘤坏死因子受体（GITR）影响Treg细胞的功能。本研究旨在评估新诊断乳腺癌女性外周血单个核细胞（PBMC）中一些重要的Treg细胞相关标志物的表达。方法：从20例乳腺癌女性患者和20例健康个体中获取新鲜PBMC。两组的PBMC在有或无植物血凝素（PHA，10μg/ml）的情况下培养32小时。从培养的PBMC中提取总RNA后，使用实时聚合酶链反应（real time-PCR）评估FOXP3、CTLA-4和GITR转录本的表达。结果：乳腺癌患者未刺激的PBMC中FOXP3、CTLA-4和GITR的mRNA表达显著高于健康对照组（分别为P<0.05、P<0.03和P<0.04）。同样，与健康个体相比，乳腺癌患者PHA刺激的PBMC中FOXP3、CTLA-4和GITR转录本的表达显著增加（分别为P<0.01、P<0.005和P<0.01）。结论：FOXP3、CTLA-4和GITR表达增加代表乳腺癌患者Treg细胞活性更高，这可能在肿瘤的发生和发展中起重要作用。
