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FUGE:一款用于教学目的的分析型超速离心机模拟软件。

FUGE: an analytical ultracentrifuge simulation for teaching purposes.

作者信息

Mynett A

机构信息

Brighton Centre for Advanced Biology, Brighton College of Technology, Department of Applied Science, UK.

出版信息

Comput Appl Biosci. 1988 Apr;4(2):249-52. doi: 10.1093/bioinformatics/4.2.249.

Abstract

An IBM-compatible microcomputer program for teaching purposes is described which stimulates the operation of a sedimentation velocity determination of a protein in an analytical ultracentrifuge using schlieren optics. The program operates in speeded-up time and simulates the major procedures which would need to be carried out to operate such an instrument. The position of the sedimenting boundary can be observed at any time during the run, and up to six 'photographs' can be recorded for subsequent analysis. Calculation of sedimentation coefficient, diffusion coefficient and mol. wt can be made from a dot-matrix printout. Ten representative proteins are stored within the program, but provision exists for user-supplied data.

摘要

本文描述了一个用于教学目的的IBM兼容微机程序,该程序可模拟使用纹影光学系统在分析超速离心机中测定蛋白质沉降速度的操作。该程序以加速时间运行,并模拟操作此类仪器所需的主要步骤。在运行过程中的任何时候都可以观察到沉降边界的位置,并且最多可以记录六张“照片”以供后续分析。沉降系数、扩散系数和分子量可以从点阵打印输出中计算得出。程序中存储了十种代表性蛋白质,但也允许用户提供数据。

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