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酶改性豆浆对脱脂乳中凝乳酶诱导凝胶的影响。

Effect of Enzyme Modified Soymilk on Rennet Induced Gelation of Skim Milk.

机构信息

Beijing Laboratory for Food Quality and Safety, Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China.

Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticides, Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China.

出版信息

Molecules. 2018 Nov 26;23(12):3084. doi: 10.3390/molecules23123084.

Abstract

In this study, soymilk was hydrolyzed to different degrees with flavourzyme, and then soymilk and enzyme modified soymilk at various levels were added to skim milk respectively, to generate a mixed gel using rennet. Rheological properties, scanning electron microscopy imaging, and physical and chemical indexes were examined to reveal the effect of enzyme modified soymilk on rennet induced gelation of skim milk. Results showed that soymilk inhibited the aggregation of skim milk, led to a decrease in storage modulus (G'), significantly increased moisture content and curd yield, and the resulting network was coarse. Enzyme modified soymilk with a molecular weight below 20 kDa led to a more uniform curd distribution, which counteracted the reduction of G' and allowed for the formation of a stronger gel. Both the moisture content and the curd yield increased with the addition of soymilk and enzyme modified soymilk, and overall the effect of adding a high degree of hydrolysis of enzyme modified soymilk was superior. Compared to untreated soymilk, the addition of a certain amount of enzyme modified soymilk resulted in a new protein structure, which would improve the texture of blend cheese.

摘要

在这项研究中,使用风味酶将豆浆水解至不同程度,然后将豆浆和不同程度酶改性的豆浆分别添加到脱脂乳中,使用凝乳酶生成混合凝胶。考察了流变特性、扫描电子显微镜成像以及物理化学指标,以揭示酶改性豆浆对脱脂乳凝乳诱导胶凝的影响。结果表明,豆浆抑制了脱脂乳的聚集,导致储能模量(G')显著降低,显著增加了水分含量和凝乳产率,且所得网络较粗糙。分子量低于 20 kDa 的酶改性豆浆导致凝乳分布更加均匀,这抵消了 G'的降低,使凝胶形成更加强劲。随着豆浆和酶改性豆浆的添加,水分含量和凝乳产率均增加,且添加高度水解的酶改性豆浆的效果总体上更优。与未处理的豆浆相比,添加一定量的酶改性豆浆会形成新的蛋白质结构,从而改善混合干酪的质地。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fee/6321578/116adfaff1f9/molecules-23-03084-g001.jpg

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