Isnaini Isnaini, Permatasari Nur, Mintaroem Karyono, Prihardina Bunga, Widodo M Aris
Departement of Pharmacology, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia. Email:
Asian Pac J Cancer Prev. 2018 Nov 29;19(11):3175-3178. doi: 10.31557/APJCP.2018.19.11.3175.
Reactive oxygen species (ROS) have various biological effects and they are non-linear in characteristic. In high oxidative stress, they may cause cytotoxicity, inhibit cell proliferation, and induce cell death in the form of apoptosis/necrosis; while in low or medium oxidative stress, ROS may cause DNA damage, cell mutation, inflammation, cell proliferation, and eventually they may induce carcinogenesis. Antioxidants are compounds with the ability to reduce ROS. Cell line MCF-7 is one of the breast cancer cell lines that is known to have small amount of antioxidant MnSOD compared to the other cell lines. Low antioxidant MnSOD level in breast cancer cell line MCF-7 leads to low concentration of hydrogen peroxide, because antioxidant MnSOD will convert radical superoxide to hydrogen peroxide. The aim of this research was to analyze oxidants and antioxidants profile in breast cancer cell line MCF-7 and their relationship with cell number. Observations were conducted for 5 days. The cell number was counted with tryphan blue method and haematometer. The concentration of radical superoxide was measured with DHE staining using LSCM tipe Olympus Fluoview FV 1000-Ver 1.7. MnSOD activity, hydrogen peroxide concentration, and catalase activity were measured with ELISA. The results showed that the longer of observation, the greater concentration of oxidants and MnSOD activity, but there was no change in catalase activity. Conclusion the increase in cancer cells number is influenced by radical superoxide.
活性氧（ROS）具有多种生物学效应，其特性呈非线性。在高氧化应激状态下，它们可能导致细胞毒性，抑制细胞增殖，并以凋亡/坏死的形式诱导细胞死亡；而在低或中度氧化应激状态下，ROS可能导致DNA损伤、细胞突变、炎症、细胞增殖，并最终可能诱导癌变。抗氧化剂是具有还原ROS能力的化合物。MCF-7细胞系是乳腺癌细胞系之一，与其他细胞系相比，已知其抗氧化剂锰超氧化物歧化酶（MnSOD）含量较少。乳腺癌细胞系MCF-7中抗氧化剂MnSOD水平较低导致过氧化氢浓度较低，因为抗氧化剂MnSOD会将超氧自由基转化为过氧化氢。本研究的目的是分析乳腺癌细胞系MCF-7中的氧化剂和抗氧化剂概况及其与细胞数量的关系。观察持续5天。用台盼蓝法和血细胞计数器对细胞数量进行计数。使用奥林巴斯Fluoview FV 1000-Ver 1.7型激光扫描共聚焦显微镜（LSCM）通过DHE染色测量超氧自由基的浓度。用酶联免疫吸附测定法（ELISA）测量MnSOD活性、过氧化氢浓度和过氧化氢酶活性。结果表明，观察时间越长，氧化剂浓度和MnSOD活性越高，但过氧化氢酶活性没有变化。结论癌细胞数量的增加受超氧自由基的影响。
