Oddó David, Stefanelli Angeles, Villarroel Alejandra, Méndez Gonzalo P
1 Pontificia Universidad Católica de Chile, Santiago, Chile.
Int J Surg Pathol. 2019 Jun;27(4):380-386. doi: 10.1177/1066896918815580. Epub 2018 Nov 29.
Granulomatous lobular mastitis is a rare disease whose origin is still unknown and shows an increase in its frequency. Morphological, microbiological, and molecular biology studies have linked this disease to lipophilic and fastidious corynebacteria, suggesting its possible infectious etiology. This series describes and reviews in detail the distinctive morphological characteristics of the bacteria present in the granulomas of this disease, the usefulness of histochemical techniques for their identification, and our proposal for a tissue quantification score for the bacteria. The MacCallum-Goodpasture method of Gram's stain turned out to be the gold standard for examination, but we also highlight the efficiency of hematoxylin and eosin stain when it is exhaustively examined as well as the Grocott stain to evaluate the bacterial pleomorphism method, which is often underutilized.
肉芽肿性小叶性乳腺炎是一种罕见疾病，其病因尚不清楚，且发病率呈上升趋势。形态学、微生物学和分子生物学研究已将该疾病与亲脂性和苛求性棒状杆菌联系起来，提示其可能的感染性病因。本系列详细描述并综述了该疾病肉芽肿中存在的细菌独特的形态特征、组织化学技术对其鉴定的实用性，以及我们提出的细菌组织定量评分方法。革兰染色的麦卡勒姆-古德帕斯特定法被证明是检查的金标准，但我们也强调了苏木精和伊红染色在全面检查时的有效性，以及用于评估细菌多形性的格罗科特染色法，该方法常未得到充分利用。
