Manose Health and Beauty Research Center, 179 Moo 10, Klong Cholprathan Road, Chiang Mai 50200, Thailand.
Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
J Nanosci Nanotechnol. 2019 Apr 1;19(4):1996-2007. doi: 10.1166/jnn.2019.15802.
Liposomes, entrapped with Job's Tears fractions, were prepared by the supercritical carbon dioxide fluid (scCO₂) technique with and without sonication. Physical characteristics, which were the particle size, zeta potential, vesicular morphology, microviscosity and bioactivities including anti-proliferative, apoptotic and antioxidative activities of the S1L-S5L liposomal systems, were investigated. The potent anti-proliferative activity with the IC value of 4.44±2.31 ug/ml in a colon cancer cell (HT-29) was observed in the S5L. S5L also showed the apoptotic activity of 4.45±0.92% in an HT-29 cell. For antioxidative activities, the S3L showed the highest free radical scavenging activity and lipid peroxidation inhibition, whereas the S4L gave the highest metal chelating activity. This study has demonstrated the potent anti-proliferative activity on an HT-29 cell of the S5L liposomal formulation prepared by the scCO₂ technique which can be further developed towards a novel anticancer drug.
采用超临界二氧化碳流体（scCO₂）技术并结合超声处理，制备了包载薏苡仁油各馏分的脂质体。考察了 S1L-S5L 脂质体系统的物理特性，包括粒径、Zeta 电位、囊泡形态、微粘度和生物活性，如增殖抑制、凋亡和抗氧化活性。在结肠癌细胞（HT-29）中，S5L 表现出 4.44±2.31ug/ml 的强增殖抑制活性。S5L 在 HT-29 细胞中还表现出 4.45±0.92%的凋亡活性。在抗氧化活性方面，S3L 显示出最高的自由基清除活性和脂质过氧化抑制作用，而 S4L 则显示出最高的金属螯合活性。本研究表明，采用 scCO₂ 技术制备的 S5L 脂质体具有较强的抗 HT-29 细胞增殖活性，可进一步开发为新型抗癌药物。
