Manosroi Aranya, Sainakham Mathukorn, Chankhampan Charinya, Manosroi Worapaka, Manosroi Jiradej
Manose Health and Beauty Research Center, Mueng, Chiang Mai 50200, Thailand; Natural Products Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University, Chiang Mai 50200, Thailand; Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Saudi J Biol Sci. 2016 Mar;23(2):248-56. doi: 10.1016/j.sjbs.2015.03.008. Epub 2015 Mar 27.
The whole seed (W), endosperm (E) and hull (H) of five cultivars of Job's tears (Coix lachryma-jobi Linn. var. ma-yuen Stapf) including Thai Black Phayao, Thai Black Loei, Laos Black Loei, Laos White Loei and Laos Black Luang Phra Bang were processed before solvent extraction by non-cooking, roasting, boiling and steaming Each part of the Job's tears was extracted by the cold and hot process by refluxing with methanol and hexane. The total of 330 extracts included 150 methanol extracts and 180 hexane extracts were investigated for anti-proliferative activity on human colon adenocarcinoma cell line (HT-29) by the sulforhodamine B (SRB) assay. The extracts which gave high anti-proliferative activity were tested for apoptotic activity by acridine orange and ethidium bromide double staining and anti-oxidative activities including free radical scavenging and lipid peroxidation inhibition activities. The extract from the hull of Thai Black Loei roasted before extracting by hot methanol (M-HTBL-R2) showed the highest anti-proliferative activity on HT-29 with the IC50 values of 11.61 ± 0.95 μg/ml, while the extract from the non-cooked hull of Thai Black Loei by cold methanol extraction (M-HTBL-N1) gave the highest apoptosis (8.17 ± 1.18%) with no necrosis. In addition, M-HTBL-R2 and M-HTBL-N1 indicated free radical scavenging activity at the SC50 values of 0.48 ± 0.12 and 2.47 ± 1.15 mg/ml, respectively. This study has demonstrated the anti-colorectal cancer potential of the M-HTBL-R2 and M-HTBL-N1 extracts.
对包括泰国黑帕尧、泰国黑黎府、老挝黑黎府、老挝白黎府和老挝黑琅勃拉邦在内的5个薏苡仁品种的全籽（W）、胚乳（E）和谷壳（H）在溶剂萃取前进行了非蒸煮、烘烤、煮沸和蒸煮处理。薏苡仁的每个部分通过甲醇和己烷回流进行冷、热提取。通过磺酰罗丹明B（SRB）测定法，对总共330种提取物（包括150种甲醇提取物和180种己烷提取物）进行了对人结肠腺癌细胞系（HT - 29）的抗增殖活性研究。对具有高抗增殖活性的提取物通过吖啶橙和溴化乙锭双重染色进行凋亡活性测试以及包括自由基清除和脂质过氧化抑制活性在内的抗氧化活性测试。热甲醇提取前烘烤的泰国黑黎府谷壳提取物（M - HTBL - R2）对HT - 29显示出最高的抗增殖活性，IC50值为11.61±0.95μg/ml，而冷甲醇提取的未蒸煮泰国黑黎府谷壳提取物（M - HTBL - N1）具有最高的凋亡率（8.17±1.18%）且无坏死现象。此外，M - HTBL - R2和M - HTBL - N1的自由基清除活性的SC50值分别为0.48±0.12和2.47±1.15mg/ml。本研究证明了M - HTBL - R2和M - HTBL - N1提取物具有抗结直肠癌的潜力。
