Kirpatovskii V I, Efremov G D, Frolova E V
N. A. Lopatkin Research Institute of Urology and Interventional Radiology, Affiliated Branch of National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia.
All-Russian Institute of Scientific and Technical Information, Russian Academy of Sciences, Moscow, Russia.
Bull Exp Biol Med. 2018 Dec;166(2):268-273. doi: 10.1007/s10517-018-4330-4. Epub 2018 Nov 28.
In experiments on white outbred male rats, a freshly removed (20 experiments) or cryopreserved (10 experiments) testicle from newborn rats (1-2 days after birth) was transplanted under the renal capsule after bilateral orchiectomy. In all experiments with transplantation of freshly removed testicle, it was engrafted. In 3 months, histological examination revealed the formation of mature seminiferous tubules, but spermatogenesis was blocked at the stage of spermatogonia; groups of proliferating Leydig cells in the loose connective tissue between the tubules were also seen. In 6 and 12 months, the status of the seminiferous tubules remained unchanged, but structures typical of the epididymis and developing vas deferens were revealed. The number of proliferating Leydig cells increased. The initially low testosterone concentration in the blood of castrated males increased significantly as soon as in 1 month after transplantation and grew up to 3 months, remaining at a level ~50% of normal. Engraftment of cryopreserved neonatal testicular tissue was observed in 60% cases, however, engrafted tissue, similar to the fresh one, retained the ability for organogenesis with the formation of mature seminiferous tubules, epididymis, and groups of proliferating Leydig cells. The dynamics of blood testosterone concentration in rats with cryopreserved and fresh transplantation was similar. Subcapsular transplantation did not adversely affect the kidneys, which was seen from normal histological structure of the kidneys and creatinine and urea concentrations in the blood.
在对白化远交系雄性大鼠进行的实验中，将新生大鼠（出生后1 - 2天）刚摘除的睾丸（20次实验）或冷冻保存的睾丸（10次实验）在双侧睾丸切除术后移植到肾被膜下。在所有移植刚摘除睾丸的实验中，睾丸均成功植入。3个月时，组织学检查显示形成了成熟的生精小管，但精子发生在精原细胞阶段受阻；在小管之间的疏松结缔组织中也可见成群增殖的Leydig细胞。6个月和12个月时，生精小管的状态保持不变，但发现了附睾和发育中的输精管的典型结构。增殖的Leydig细胞数量增加。去势雄性大鼠血液中最初较低的睾酮浓度在移植后1个月就显著升高，并持续到3个月，维持在正常水平的约50%。60%的病例观察到冷冻保存的新生睾丸组织成功植入，然而，植入的组织与新鲜组织相似，保留了器官发生的能力，形成了成熟的生精小管、附睾和成组的增殖Leydig细胞。冷冻保存和新鲜移植大鼠血液中睾酮浓度的变化动态相似。从肾脏的正常组织结构以及血液中肌酐和尿素浓度可以看出，被膜下移植对肾脏没有不良影响。
