Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
Mol Biol Rep. 2019 Feb;46(1):551-567. doi: 10.1007/s11033-018-4508-x. Epub 2018 Nov 28.
Interferon regulatory factors (IRF-1 and IRF-2) are transcription factors of IRF-family that regulate expression of genes for cytokines, chemokines and growth factors in mammalian cells. IRF-1 and IRF-2 play crucial roles in the differentiation of bone marrow cells for immune response. Bone marrow (BM) is the soft lymphoid organ that contains many types of stem cells and produces different types of cells of the blood and immune system. Genetic alterations and damage of the bone marrow cells can lead to different types of blood and immune system-related diseases including anemia and cancer. We have studied the expression of IRF-1 and IRF-2 during radiation-induced damage and regeneration of bone marrow cells after transplantation of freshly isolated bone marrow cells in the mouse. Cell cycle analysis, colony forming unit-fibroblast (CFU-F) assay and bone marrow histology showed that after radiation-induced damage, the bone marrow transplantation resulted in regeneration of the bone marrow up to 24-35% recovery. Real-time quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) for the mRNA expression showed that IRF-1 and IRF-2 were expressed at higher levels in the bone marrow cells of the irradiated (4.34× fold for IRF-1, and 3.87× fold for IRF-2) compared to control and transplanted (1.13× fold for IRF-1, and 1.12× fold IRF-2) mice and immuno-fluorescence analysis for the protein expression showed that IRF-1 and IRF-2 were expressed at higher levels in the bone marrow cells of the irradiated (2.12× fold for IRF-1 and 1.71× fold for IRF-2) compared to control and transplanted (1.73× fold for IRF-1 and 1.21× fold for IRF-2) mice. Thus, IRF-1 and IRF-2 are sensitive and responsive to radiation-induced damage in the bone marrow cells and may also be involved in the bone marrow regeneration process.
干扰素调节因子（IRF-1 和 IRF-2）是 IRF 家族的转录因子，调节哺乳动物细胞中细胞因子、趋化因子和生长因子的基因表达。IRF-1 和 IRF-2 在骨髓细胞免疫反应的分化中发挥着至关重要的作用。骨髓（BM）是一种柔软的淋巴器官，其中包含多种类型的干细胞，并产生不同类型的血液和免疫系统细胞。骨髓细胞的遗传改变和损伤可导致不同类型的血液和免疫系统相关疾病，包括贫血和癌症。我们研究了在新鲜分离的骨髓细胞移植后，辐射诱导的骨髓细胞损伤和再生过程中 IRF-1 和 IRF-2 的表达。细胞周期分析、集落形成单位-成纤维细胞（CFU-F）测定和骨髓组织学显示，在辐射诱导损伤后，骨髓移植导致骨髓再生，恢复率高达 24-35%。实时定量逆转录聚合酶链反应（Q-RT-PCR）检测 mRNA 表达显示，与对照组和移植组相比（IRF-1 为 1.13 倍，IRF-2 为 1.12 倍），受照射的骨髓细胞中 IRF-1 和 IRF-2 的表达水平更高（IRF-1 为 4.34 倍，IRF-2 为 3.87 倍），免疫荧光分析显示，与对照组和移植组相比（IRF-1 为 1.73 倍，IRF-2 为 1.21 倍），受照射的骨髓细胞中 IRF-1 和 IRF-2 的表达水平更高（IRF-1 为 2.12 倍，IRF-2 为 1.71 倍）。因此，IRF-1 和 IRF-2 对骨髓细胞的辐射损伤敏感且有反应性，也可能参与骨髓再生过程。
