Giordano Braden C, Siefert Ronald, Collins Greg E
Chemistry Division, U.S. Naval Research Laboratory, Washington, DC, USA.
Chemistry Department , United States Naval Academy, Annapolis, MD, USA.
Methods Mol Biol. 2019;1906:87-97. doi: 10.1007/978-1-4939-8964-5_6.
Micellar electrokinetic chromatography (MEKC) is a mode of capillary electrophoresis that allows for the separation of neutral molecules in an electric field. Typically, neutral molecules move with electroosmotic flow (EOF) or bulk flow during electrophoretic separations resulting in no temporal resolution between mixtures of neutral analytes. Inclusion of surfactant micelles in the separation buffer allows for the separation of neutral analytes from one another through association with the micelle. Here we outline the implementation of MEKC for the separation of neutral molecules using a mixture of nitroaromatic explosives and their degradation products serving as a test analyte mixture.
胶束电动色谱法（MEKC）是一种毛细管电泳模式，可在电场中分离中性分子。通常，在电泳分离过程中，中性分子随电渗流（EOF）或总体流动移动，导致中性分析物混合物之间没有时间分辨率。在分离缓冲液中加入表面活性剂胶束可通过与胶束结合来分离中性分析物。在此，我们概述了使用硝基芳香族炸药及其降解产物的混合物作为测试分析物混合物，采用胶束电动色谱法分离中性分子的方法。
