College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
Lotte R&D Center, Seoul, South Korea.
J Sci Food Agric. 2019 Apr;99(6):3045-3056. doi: 10.1002/jsfa.9518. Epub 2019 Jan 22.
This study investigated the in vivo prophylactic effect of probiotic chocolate on constipation. Rats were administered chocolate containing 2.5 × 10 CFU g of probiotics daily for 4 weeks and treated with loperamide (5 mg kg ) daily at the fourth week of treatment.
Probiotic chocolate treatment significantly (P < 0.05) increased the intestinal motility, colon length, fecal moisture content and number of excreted fecal pellets in constipated rats. Moreover, quantitative real-time polymerase chain reaction data and histological images also revealed that both probiotic chocolate LYC and BB12 treatments were capable of upregulating the mRNA expression levels of colonic ZO-1, occludin and AQP8, leading to the maintenance of the defensive barrier function in the constipated rats compared with the negative controls. Interestingly, these treatments also modulated gut bacterial populations by increasing the abundance levels of Lactobacillus and Bifidobacterium, as well as reducing the abundance level of Enterobacteriaceae.
The present study demonstrated that probiotic chocolate LYC and BB12 could potentially be used as alternative agents for prophylactic constipation. © 2018 Society of Chemical Industry.
本研究调查了益生菌巧克力对便秘的体内预防作用。大鼠每天给予含有 2.5×10 CFU/g 益生菌的巧克力，连续 4 周，并在治疗的第 4 周每天给予洛哌丁胺（5mg/kg）。
益生菌巧克力治疗可显著（P<0.05）增加便秘大鼠的肠道蠕动、结肠长度、粪便水分含量和粪便颗粒排出数。此外，实时定量聚合酶链反应数据和组织学图像还表明，益生菌巧克力 LYC 和 BB12 治疗均能上调结肠 ZO-1、occludin 和 AQP8 的 mRNA 表达水平，与阴性对照组相比，维持便秘大鼠的防御屏障功能。有趣的是，这些治疗还通过增加乳酸菌和双歧杆菌的丰度水平以及降低肠杆菌科的丰度水平来调节肠道细菌种群。
本研究表明，益生菌巧克力 LYC 和 BB12 可作为预防便秘的替代药物。© 2018 化学工业协会。
