Osama Eman, Galal Azza A A, Abdalla Hany, El-Sheikh Sawsan M A
Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Andrologia. 2019 Apr;51(3):e13214. doi: 10.1111/and.13214. Epub 2018 Nov 29.
This study was carried out to investigate the potential effects of Chlorella vulgaris (CV) on testicular function and oxidant/antioxidant status in normal and deltamethrin-intoxicated rats. Forty adult male rats were drenched either with normal saline, CV (50 mg/kg), deltamethrin (DM) (3 mg/kg), or CV combined with DM, daily for 8 weeks. At the end of the protocol, the epididymal sperm quality was evaluated and the testicular superoxide dismutase (SOD), catalase enzyme (CAT) and malondialdehyde (MDA), and the serum testosterone levels were estimated. Normal rats treated with CV showed a significant increase in the total sperm number/epididymal tail, testicular SOD, and CAT levels with a significant decrease in the testicular MDA. Deltamethrin intoxication significantly decreased the proportions of motile and live sperm, the testosterone concentration, the testicular SOD and CAT levels, whereas it significantly increased the proportion of abnormal sperm and the testicular MDA. Chlorella vulgaris treatment significantly ameliorated the adverse effects of DM-intoxication and restored most of the parameters to levels that are comparable to those of the control group. In conclusion, CV administration improved the testicular function of normal rats and ameliorated the effect of severe oxidative stress conditions.
本研究旨在探讨普通小球藻（CV）对正常和溴氰菊酯中毒大鼠睾丸功能及氧化/抗氧化状态的潜在影响。40只成年雄性大鼠每天分别灌胃生理盐水、CV（50毫克/千克）、溴氰菊酯（DM）（3毫克/千克）或CV与DM的组合，持续8周。实验结束时，评估附睾精子质量，并测定睾丸超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）以及血清睾酮水平。用CV处理的正常大鼠附睾尾部精子总数、睾丸SOD和CAT水平显著增加，并伴有睾丸MDA显著降低。溴氰菊酯中毒显著降低了活动精子和活精子的比例、睾酮浓度、睾丸SOD和CAT水平，而异常精子比例和睾丸MDA显著增加。普通小球藻处理显著改善了DM中毒的不良反应，并使大多数参数恢复到与对照组相当的水平。总之，给予CV可改善正常大鼠的睾丸功能，并减轻严重氧化应激条件的影响。
