Abdallah Fatma Ben, Fetoui Hamadi, Zribi Nassira, Fakhfakh Feiza, Keskes Leila
Laboratory of Histology, Embryology, and Reproductive Biology, University of Sfax, Sfax, Tunisia.
Toxicol Ind Health. 2012 Aug;28(7):639-47. doi: 10.1177/0748233711420470. Epub 2011 Oct 24.
The synthetic pyrethroids are expected to cause deleterious effects on most of the organs and especially on the male reproductive system. The current study was performed to assess the adverse effect of lambda cyhalothrin (LC) on reproductive organs and fertility in male rats and to evaluate the protective role of caffeic acid phenethyl ester (CAPE) in alleviating the detrimental effect of LC on male fertility. A total of 48 male rats were divided into 4 groups (12 rats each): control group received distilled water ad libitum and 1 ml of vehicle solution given intraperitoneally (i.p.); CAPE-treated group received a single i.p. dose of CAPE (10 μmol kg⁻¹ day⁻¹); LC-treated group received 668 ppm of LC through drinking water; and CAPE + LC-treated group received an i.p. injection of CAPE (10 μmol kg⁻¹ day⁻¹) 12 h before the LC administration. The experiment was conducted for 10 consecutive weeks. LC caused a significant increase in testicular malondialdehyde, catalase, superoxide dismutase, glutathione-S-transferase activities, and sperm abnormalities and a significant reduction in testicular glutathione concentration, sperm count, sperm motility, and a live sperm percentage. Conversely, treatment with CAPE improved the reduction in the sperm characteristics, LC-induced oxidative damage of testes and the testicular histopathological alterations. Results indicate that LC exerts significant harmful effects on the male reproductive system and that CAPE reduced the deleterious effects of LC on male fertility.
合成拟除虫菊酯预计会对大多数器官产生有害影响，尤其是对雄性生殖系统。进行本研究以评估高效氯氟氰菊酯（LC）对雄性大鼠生殖器官和生育能力的不良影响，并评估咖啡酸苯乙酯（CAPE）在减轻LC对雄性生育能力的有害影响方面的保护作用。总共48只雄性大鼠被分为4组（每组12只）：对照组自由饮用蒸馏水，并腹腔注射1毫升赋形剂溶液；CAPE处理组腹腔注射单次剂量的CAPE（10 μmol kg⁻¹ 天⁻¹）；LC处理组通过饮用水摄入668 ppm的LC；CAPE + LC处理组在给予LC前12小时腹腔注射CAPE（10 μmol kg⁻¹ 天⁻¹）。实验连续进行10周。LC导致睾丸丙二醛、过氧化氢酶、超氧化物歧化酶、谷胱甘肽-S-转移酶活性显著增加，精子异常显著增加，同时睾丸谷胱甘肽浓度、精子计数、精子活力和活精子百分比显著降低。相反，CAPE处理改善了精子特征的降低、LC诱导的睾丸氧化损伤和睾丸组织病理学改变。结果表明，LC对雄性生殖系统产生显著有害影响，而CAPE降低了LC对雄性生育能力的有害影响。
