Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
Department of Obstetrics and Gynaecology, Faculty of Medicine, University Malaysia, 50603, Kuala Lumpur, Malaysia.
Proteomics Clin Appl. 2019 May;13(3):e1800153. doi: 10.1002/prca.201800153. Epub 2018 Nov 29.
Endometriosis affects about 4% of women in the reproductive age and is associated with subfertility. The aim of the present study is to examine the integrated quantitative proteomic profile of eutopic endometrium and serum from women with endometriosis compared to controls in order to identify candidate disease-specific serological markers.
Eutopic endometrium and serum from patients with endometriosis (n = 8 for tissue and n = 4 for serum) are, respectively, compared to endometrium and serum from females without endometriosis (n = 8 for tissue and n = 4 for serum) using a shotgun quantitative proteomics method. All study participants are at the proliferative phase of their menstrual cycle.
At the tissue and serum level, 1214 and 404 proteins are differentially expressed (DEPs) in eutopic endometrium and serum, respectively, of women with endometriosis versus controls. Gene ontology analysis shows that terms related to immune response/inflammation, cell adhesion/migration, and blood coagulation are significantly enriched in the DEPs of eutopic endometrium, as well as serum. Twenty-one DEPs have the same trend of differential expression in both matrices and can be further examined as potential disease- and tissue-specific serological markers of endometriosis.
The present integrated proteomic profiling of eutopic endometrium and serum from women with endometriosis identify promising serological markers that can be further validated in larger cohorts for the minimally invasive diagnosis of endometriosis.
子宫内膜异位症影响约 4%的育龄妇女，与不孕有关。本研究旨在比较子宫内膜异位症患者的在位子宫内膜和血清与对照组的综合定量蛋白质组学特征，以确定候选的疾病特异性血清标志物。
采用shotgun 定量蛋白质组学方法，分别比较子宫内膜异位症患者的在位子宫内膜和血清（组织 n=8，血清 n=4）与无子宫内膜异位症的女性的子宫内膜和血清（组织 n=8，血清 n=4）。所有研究参与者均处于月经周期的增殖期。
在组织和血清水平上，与对照组相比，子宫内膜异位症患者的在位子宫内膜和血清中分别有 1214 种和 404 种蛋白表达差异（DEPs）。基因本体论分析表明，与免疫反应/炎症、细胞黏附和迁移以及血液凝固相关的术语在在位子宫内膜和血清的 DEPs 中显著富集。21 种 DEPs 在两种基质中的差异表达趋势相同，可进一步作为子宫内膜异位症潜在的疾病和组织特异性血清标志物进行检查。
本研究对子宫内膜异位症患者的在位子宫内膜和血清进行了综合蛋白质组学分析，确定了有前途的血清标志物，可在更大的队列中进一步验证，用于子宫内膜异位症的微创诊断。
