Beckman Research Institute at City of Hope, Duarte, CA, USA.
Faculty of Health Sciences, University of Southampton, Southampton, UK.
Nutr Diabetes. 2020 Jan 14;10(1):2. doi: 10.1038/s41387-019-0105-x.
Epidemiological data suggest that pre-eclampsia (PE) is associated with an increased risk of post-delivery metabolic dysregulation. The aim of the present case-control observational study was to examine the global plasma proteomic profile 1 year postpartum in women who developed PE during pregnancy (n = 5) compared to controls (n = 5), in order to identify a novel predictive marker linking PE with long-term metabolic imbalance. Key findings were verified with enzyme-linked immunosorbent assay (ELISA) in a separate cohort (n = 17 women with PE and n = 43 controls). One hundred and seventy-two proteins were differentially expressed in the PE vs. control groups. Gene ontology analysis showed that Inflammatory|Immune responses, Blood coagulation and Metabolism were significantly enriched terms. CD14, mapping to the inflammatory response protein network, was selected for verification based on bibliographic evidence. ELISA measurements showed CD14 to be significantly increased 1 year postpartum in women with PE during pregnancy compared to controls [PE group (median ± SD): 296.5 ± 113.6; control group (median ± SD): 128.9 ± 98.5; Mann-Whitney U test p = 0.0078]. Overall, the identified proteins could provide insight into the long-term disease risk among women with PE during pregnancy and highlight the need for their postpartum monitoring. CD14 could be examined in larger cohorts as a predictive marker of insulin resistance and type II diabetes mellitus among women with PE.
流行病学数据表明，先兆子痫（PE）与产后代谢失调风险增加有关。本病例对照观察性研究的目的是在产后 1 年检查患有妊娠 PE 的妇女（n=5）与对照组（n=5）的全血浆蛋白质组谱，以确定一种将 PE 与长期代谢失衡联系起来的新预测标志物。关键发现通过在另一个队列中（PE 组 n=17 例，对照组 n=43 例）进行酶联免疫吸附测定（ELISA）进行了验证。PE 组与对照组相比，有 172 种蛋白质表达存在差异。GO 分析显示，炎症|免疫反应、血液凝固和代谢是显著富集的术语。基于文献证据，选择 CD14（映射到炎症反应蛋白网络）作为验证的候选标志物。ELISA 测量显示，妊娠 PE 妇女在产后 1 年时 CD14 明显高于对照组[PE 组（中位数±SD）：296.5±113.6；对照组（中位数±SD）：128.9±98.5；Mann-Whitney U 检验，p=0.0078]。总体而言，所鉴定的蛋白质可以深入了解妊娠 PE 妇女的长期疾病风险，并强调了对其产后监测的必要性。可以在更大的队列中检查 CD14，作为 PE 妇女胰岛素抵抗和 II 型糖尿病的预测标志物。
