Marianowski Piotr, Szymusik Iwona, Malejczyk Jacek, Hibner Michael, Wielgos Miroslaw
1st Department of Obstetrics and Gynecology, Medical University of Warsaw, Poland.
Department of Histology and Embryology, Center for biostructure Research, Medical University of Warsaw, Poland.
Neuro Endocrinol Lett. 2013;34(7):717-21.
The present study aimed at performing proteomic analysis of matched eutopic endometrium and ovarian endometrioid cysts from women with endometriosis in order to discover any abnormal protein expression related to the disease.
The study included 8 women with stage III/IV endometriosis according to revised American Fertility Society (rAFS) classification and one woman with no signs of the disease as a reference. Proteomic analysis was performed using a novel isobaric tag-based methodology for relative and absolute peptide quantification (iTRAQ) coupled with multidimensional liquid chromatography and tandem mass spectrometry.
The selection of 419 proteins was found in all endometriosis specimens. Using normal eutopic endometrium from woman without endometriosis as a reference, some proteins expressions were significantly increased in all endometriosis samples. They included collagen α1(XIV), calmodulin, collagen α(VI), plexin, integrin αVβ3, transgelin, desmin, and vimentin. The comparison of these proteins' expression in paired eutopic and ovarian endometriosis samples has revealed that only vimentin was significantly increased in ovarian endometrioma.
It was confirmed that endometriosis is associated with different expression of proteins in endometriotic samples. Nevertheless, further studies seem to be necessary as they may reveal possible markers that would be useful in clinical diagnosis of the disease.
本研究旨在对子宫内膜异位症患者的配对在位内膜和卵巢子宫内膜样囊肿进行蛋白质组学分析，以发现与该疾病相关的任何异常蛋白表达。
该研究纳入了8例根据修订后的美国生育协会（rAFS）分类为III/IV期子宫内膜异位症的女性，以及1例无该病体征的女性作为对照。蛋白质组学分析采用一种基于新型等压标签的相对和绝对肽定量方法（iTRAQ），结合多维液相色谱和串联质谱法进行。
在所有子宫内膜异位症标本中发现了419种蛋白质的选择。以无子宫内膜异位症女性的正常在位内膜作为对照，在所有子宫内膜异位症样本中，一些蛋白质的表达显著增加。它们包括胶原蛋白α1（XIV）、钙调蛋白、胶原蛋白α（VI）、丛状蛋白、整合素αVβ3、转胶蛋白、结蛋白和波形蛋白。这些蛋白质在配对的在位内膜和卵巢子宫内膜异位症样本中的表达比较显示，只有波形蛋白在卵巢子宫内膜瘤中显著增加。
证实子宫内膜异位症与子宫内膜异位症样本中蛋白质的不同表达有关。然而，似乎有必要进行进一步的研究，因为它们可能揭示在该疾病临床诊断中有用的潜在标志物。
