Carlson K H, Bourne W M
Department of Ophthalmology, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
Cornea. 1988;7(3):183-9.
The endothelium maintains corneal deturgescence in part by serving as a barrier to fluid movement into the cornea. This barrier function is estimated by measuring the permeability of the endothelium to small, nontoxic, fluorescent molecules such as fluorescein and carboxy-fluorescein. After topical or systemic application of the dye, its concentration in the cornea and the anterior chamber is measured noninvasively at intervals over several hours with a fluorophotometer. Endothelial permeability to the fluorescent dye is calculated from the changes in its concentration in the cornea and anterior chamber over time. The permeability of the endothelium to the small fluorescent molecules is assumed to be proportional to its permeability to water, and thus provides an estimate of the barrier function of the endothelium. This paper reviews the theory and technique of clinical fluorophotometry and discusses published data concerning the effects on endothelial permeability of aging, contact lens wear, medication, and surgery.
内皮细胞部分通过充当阻止液体进入角膜的屏障来维持角膜的消肿状态。这种屏障功能是通过测量内皮细胞对诸如荧光素和羧基荧光素等小的、无毒的荧光分子的通透性来评估的。在局部或全身应用染料后，使用荧光光度计在数小时内定期无创测量其在角膜和前房中的浓度。根据其在角膜和前房中的浓度随时间的变化来计算内皮细胞对荧光染料的通透性。假定内皮细胞对小荧光分子的通透性与其对水的通透性成正比，因此可提供内皮细胞屏障功能的估计值。本文综述了临床荧光光度测定法的理论和技术，并讨论了已发表的有关衰老、佩戴隐形眼镜、药物和手术对内皮细胞通透性影响的数据。
