FitzGerald T J, Anklesaria P, Le D, Sakakeeny M A, Kase K, Greenberger J S
Department of Radiation Oncology, University of Massachusetts Medical Center, Worcester 01605.
Exp Hematol. 1988 Nov;16(10):820-6.
The x-irradiation biology of supportive stromal cells of the bone marrow microenvironment was investigated by using cloned permanent cell lines that were established from hematopoietically active murine long-term bone marrow cultures. X-irradiation survival curves were derived for each cell line at either 120 cGy/min or clinical low dose rate (LDR) (5 cGy/min) that is used in total body irradiation protocols prior to bone marrow transplantation. Four cell lines, MBA-1, MBA-13, 14F2.1, and D2XRII (Group I) demonstrated a significant increase in D0 at 5 cGy/min (280 cGy, 270 cGy, 210 cGy, and 240 cGy, respectively) compared to 120 cGy/min (215 cGy, 210 cGy, 157 cGy, and 210 cGy, respectively) (p less than 0.05). In contrast, three other clonal cell lines, +/+ #2 cl 4, S1d #3, and GPI alpha-1 (Group II) showed no significant dose rate dependent change in D0 at 5 cGy/min (164 cGy, 174 cGy, and 159 cGy, respectively) compared to 120 cGy/min (159 cGy, 167 cGy, and 143 cGy, respectively). Group I and II cell lines could not be distinguished by differences in synthesis of extracellular matrix proteins including laminin, collagen types I and IV, or histochemically detectable enzymes. Three Group I lines (MBA-1, MBA-13, and D2XRII) and one Group II line, Sld #3, showed decreased support capacity for cocultivated hematopoietic stem cells in vitro. All seven lines had detectable polyA+ mRNA for monocyte colony-stimulating factor (M-CSF) as detected by molecular hybridization; one had detectable levels of polyA+ mRNA for granulocyte-macrophage colony-stimulating factor (GM-CSF) (D2XRII); one, detectable levels of polyA+ mRNA for interleukin 1 (IL-1); and none of the seven had detectable polyA+ mRNA for granulocyte colony-stimulating factor (G-CSF) or IL-3 (multi-CSF). The data indicate that some cells of the hematopoietic microenvironment may not be selectively protected by clinical low dose rate irradiation.(ABSTRACT TRUNCATED AT 250 WORDS)
利用从具有造血活性的小鼠长期骨髓培养物中建立的克隆永久细胞系，研究了骨髓微环境支持性基质细胞的X射线辐射生物学。分别以120 cGy/分钟或临床低剂量率（LDR）（5 cGy/分钟）得出每个细胞系的X射线辐射存活曲线，临床低剂量率用于骨髓移植前的全身照射方案。四个细胞系，MBA - 1、MBA - 13、14F2.1和D2XRII（第一组）在5 cGy/分钟时的D0值（分别为280 cGy、270 cGy、210 cGy和240 cGy）相比120 cGy/分钟（分别为215 cGy、210 cGy、157 cGy和210 cGy）有显著增加（p小于0.05）。相比之下，其他三个克隆细胞系，+/ + #2 cl 4、S1d #3和GPI alpha - 1（第二组）在5 cGy/分钟时的D0值（分别为164 cGy、174 cGy和159 cGy）相比120 cGy/分钟（分别为159 cGy、167 cGy和143 cGy）未显示出显著的剂量率依赖性变化。第一组和第二组细胞系无法通过细胞外基质蛋白（包括层粘连蛋白、I型和IV型胶原蛋白）的合成差异或组织化学可检测的酶来区分。三个第一组细胞系（MBA - 1、MBA - 13和D2XRII）和一个第二组细胞系Sld #3在体外对共培养的造血干细胞的支持能力下降。通过分子杂交检测，所有七个细胞系均有可检测到的单核细胞集落刺激因子（M - CSF）的多聚腺苷酸加尾mRNA（polyA + mRNA）；一个细胞系有可检测到的粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）的多聚腺苷酸加尾mRNA水平（D2XRII）；一个细胞系有可检测到的白细胞介素1（IL - 1）的多聚腺苷酸加尾mRNA水平；七个细胞系中没有一个有可检测到的粒细胞集落刺激因子（G - CSF）或IL - 3（多能集落刺激因子）的多聚腺苷酸加尾mRNA。数据表明，造血微环境的一些细胞可能不会被临床低剂量率照射选择性保护。（摘要截断于250字）
