Petrenko V A, Kipriyanov S M, Boldyrev A N, Pozdnyakov P I
All-Union Institute of Molecular Biology, Kol'tsovo, Novosibirsk Region, USSR.
FEBS Lett. 1988 Sep 26;238(1):109-12. doi: 10.1016/0014-5793(88)80236-9.
A new approach to induce directed mutations in genes of study through simple cotransfection of E. coli cells by the mixture of primer and template was developed. This method is based on the use of synthetic phosphotriester analogues of oligonucleotides as site-specific mutagenic primers. The achieved yield of mutant clones was 2-3%.
通过将引物和模板的混合物简单共转染大肠杆菌细胞，开发出了一种在研究基因中诱导定向突变的新方法。该方法基于使用寡核苷酸的合成磷酸三酯类似物作为位点特异性诱变引物。获得的突变克隆产率为2%-3%。
